REGULATION OF FGF EXPRESSION DURING RETINAL DETACHMENT

视网膜脱离过程中 FGF 表达的调节

• 批准号: 2710920
• 负责人: Leonard Martin Hjelmeland
• 金额: $ 29.5万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2710920
• 关键词: apical membrane; basement membrane; cats; cell adhesion; cell adhesion molecules; cell cycle; cellular polarity; confocal scanning microscopy; fibroblast growth factor; gene expression; genetic regulation; growth factor receptors; human tissue; immunocytochemistry; in situ hybridization; pathologic process; phosphorylation; protein tyrosine kinase; proteoglycan; receptor binding; retina detachment; retinal pigment epithelium; western blottings

DESCRIPTION: (Adapted from applicant's abstract) The RPE plays a critical role in maintaining the viability of retinal neurons. This grant tests the hypothesis that FGFs and their receptors are segregated on the apical and basal surfaces of the RPE cells, and that loss of this polarization results in their proliferation. There are three specific aims, each with individual objectives. First, is the release of individual FGFs polarized in monolayers of human RPE in vitro. Second, are the distribution of high and low affinity FGF receptors polarized in human RPE monolayers. Third, do cell adhesion molecules regulate basic FGF gene expression.

描述：（改编自申请人的摘要）RPE起着 在维持视网膜神经元的活力中起着关键作用。 这 格兰特检验了FGFs及其受体是分离的假设 在RPE细胞的顶面和底面上， 两极分化导致其扩散。 有三个具体的 每个人都有自己的目标，每个人都有自己的目标。 第一，释放 体外人RPE单层中单个FGFs发生极化。 第二、 高亲和力和低亲和力FGF受体的分布是否极化 在人类RPE单层中。 第三，细胞粘附分子是否调节 碱性成纤维细胞生长因子基因表达。