RETROGRADE TRANSPORT/NUCLEAR IMPORT PATHWAY IN NEURONS

神经元中的逆行运输/核输入途径

• 批准号: 2037665
• 负责人: RICHARD T AMBRON
• 金额: $ 19.72万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-12-13 至 1998-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2037665
• 关键词: Aplysia; axon; biological signal transduction; enzyme linked immunosorbent assay; gel electrophoresis; laboratory rat; nerve /myelin protein; nerve endings; nerve injury; nervous system regeneration; neural information processing; neuronal transport; protein purification; protein signal sequence; soma

DESCRIPTION: Studies on regeneration and retrograde transport in Aplysia neurons identified a set of retrogradely transported proteins which contain a nuclear import signal. This signal peptide sequence is homologous to the nuclear import sequences found in a variety of other proteins, such as the S40 large T-antigen and exogenous proteins with this sequence attached are subject to both retrograde transport and nuclear import when introduced into the axon. Proteins containing such sequences have now been obtained and identified in rat. These proteins are thought to represent prototypes for the elusive nerve injury signal which is thought to mediate the cell body response following nerve injury. The experiments in this application are intended to characterize endogenous proteins containing these sequences and their associated polypeptides.The biology of these proteins in Aplysia and rat nerve will be explored in the hopes of providing insights into the cell body response after nerve injury and identifying the underlying molecular mechanisms.

描述：研究再生和逆行运输