INTERACTIVE LIVE CELL AND TISSUE ANALYSIS SYSTEM

交互式活细胞和组织分析系统

• 批准号: 2547416
• 负责人: Michel Nederlof
• 金额: $ 9.98万
• 依托单位: QED IMAGING, INC.
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-23 至 1999-12-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2547416
• 关键词: biomedical automation; biomedical equipment development; cell population study; charge coupled device camera; computer data analysis; computer program /software; computer system design /evaluation; cytology; digital imaging; fluorescence microscopy; image processing; video microscopy

Our main objective is to improve live cell and tissue R&D over a broad range of applications by designing, constructing, and marketing a high speed, interactive, live cell and tissue imaging microscope system with dedicated user-friendly application software. The growth in imaging live cell science and biotechnology has created a significant control and information processing problem that can now be solved by a combination of technologies: a fast, high-resolution digital cooled CCD camera, an optically efficient fluorescence microscope with application-specific automation, and a multiprocessor desktop computer running highly efficient and flexible core software for control, image acquisition, processing, analysis and display. Our specific aims for phase I are to l) build and test a prototype basic system, 2) expand the prototype with a non-invasive 3D VEC monitoring mode, 3) demonstrate the system as a functional live cell study tool, 4) specify the complete system for phase II, 5) write the phase I report. PROPOSED COMMERCIAL APPLICATIONS: The end product will be used to facilitate and expand the analysis of living cells and tissues and will be used in both clinical and research settings. Examples include: developmental biology, cell biology, toxicology, drug development. The system will also have important clinical and research applications in areas such as tissue engineering, or transplant technology. The end product would be useful to researchers at universities, biotechnology labs, Government facilities and eventually to clinicians at hospital.

我们的主要目标是在广泛的范围内改善活细胞和组织的研发 通过设计、构建和营销高的应用范围 高速、交互式、活细胞和组织成像显微镜系统 专用的用户友好的应用软件。 活细胞成像科学和生物技术的发展创造了 现在可以解决的重大控制和信息处理问题 通过技术组合来解决：快速、高分辨率的数字 冷却 CCD 相机，光学高效荧光显微镜 特定于应用程序的自动化和多处理器台式计算机 运行高效、灵活的控制、图像核心软件 采集、处理、分析和显示。 我们第一阶段的具体目标是 l) 构建并测试基本原型 系统，2) 通过非侵入式 3D VEC 监测扩展原型 模式，3) 演示系统作为功能性活细胞研究工具，4) 指定第二阶段的完整系统，5）编写第一阶段报告。 拟议的商业应用： 最终产品将用于促进和扩大分析 活细胞和组织将用于临床和研究 设置。例子包括：发育生物学、细胞生物学、 毒理学、药物开发。该系统还将具有重要 组织工程等领域的临床和研究应用， 或移植技术。最终产品对研究人员有用 在大学、生物技术实验室、政府设施，并最终 给医院的临床医生。