AUTOMATED MICROCHIP DNA ANALYSIS SYSTEM

本项目将开发一种自动DNA分析微芯片系统 它将在微通道中加载、运行和检测DNA样本， 微芯片 该系统将应用于单核苷酸的分析 多态性（SNP）、基因型片段和读取DNA序列，所有 以非常高的吞吐率。该系统将由微芯片和 目前正在开发的机器人仪器。 在一个典型 应用时，仪器将从微量滴定板中转移样品 到微芯片上的样品装载口。 装载的微芯片将 移动到与探测器下的电极对接。 样品将 将它们注入分离微通道中， 检测器，在其中测量荧光。 微芯片将 包含用于样品注入和分离的微通道。同相 我，我们计划描述现有的原型，并扩展其 功能. 我们将设计和制造多达48个微芯片， 用于DNA测序的通道。 该系统将使用M13进行测试 对照组和测试真实世界测序样品组， 包括M13克隆、质粒和PCR片段。 拟定商业应用： 该项目将开发一个非常高通量的DNA分析系统， 使用基于微芯片的格式进行DNA测序和基因分型。 这 系统将在生物医学研究、基础研究、 研究和应用领域，如DNA诊断，法医学，动物 植物分类和人类识别。 客户将 基因组中心，制药和生物技术公司， 大学和诊断实验室。

This project will develop an Automated DNA Analysis Microchip System that will load, run, and detect DNA samples in microchannels on microchips. The system will be applied to analyze single nucleotide polymorphisms (SNPs), genotype fragments, and read DNA sequences, all at very high throughput rates. The system will consist of microchips and a robotic instrument currently under development. In a typical application, the instrument will transfer samples from microtiter plates to sample loading ports on the microchips. The loaded microchips will be moved to dock with electrodes under a detector. The samples will them be injected into separation microchannels and electrophoresed past a detector where the fluorescence is measured. The microchips will contain microchannels for both sample injection and separation. In Phase I, we plan to characterize the existing prototype and extend its functionality. We will design and build microchips with up to 48 channels for DNA sequencing. The system will be tested with M13 controls and with panels of test real-world sequencing samples, including M13 clones, plasmids, and PCR fragments. PROPOSED COMMERCIAL APPLICATION: The project will develop a very high throughput DNA analysis system for DNA sequencing and genotyping using a microchip-based format. This system will have widespread applications in biomedical research,, basic research and in applied areas such as DNA diagnostics, forensics, animal and plant typing, and human identification. The customers will be genome centers, pharmaceutical and biotechnology companies, universities, and diagnostic laboratories.