DEVELOPMENT OF OPTICAL DEVICE FOR USE IN DRUG DISCOVERY

开发用于药物发现的光学器件

• 批准号: 2039223
• 负责人: JESSE BAUMGOLD
• 金额: $ 9.74万
• 依托单位: RECEPTOR BIOLOGY, INC.
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-15 至 1998-10-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2039223
• 关键词: biomedical equipment development; calcium ion; drug screening /evaluation; fluorescence

DESCRIPTION (Investigator's Abstract): This proposal addresses the Molecular Neurobiology interest of NINDS from the latest Omnibus solicitation. A high throughput, fluorescence based technique to measure receptor mediated cellular responses is proposed. The goal is to develop an optical probe capable of measuring intracellular Ca2+ signals reported by fluorescent dyes for high volume application such as screening a number of synthetic or natural compounds. A number of reporter dyes are now available to measure intracellular Ca2+ signals following surface membrane receptor activation and methods exist to test if these signals are transduced by inotropic or metabotropic mechanisms. The optical probe design will incorporate efficient light throughput, cell perfusion and fast data acquisition. The commercial application of the development is that a rapid, inexpensive and quantitative assay will become available to be offered as a contract service by Receptor Biology to pharmaceutical companies, government and universities to screen novel synthetic and naturally occurring compounds in the search for novel neuro-active pharmaceuticals. The major advantage of the method include: 1) independence from radioisotopes; 2) independence from laboratory animals; 3) rapidity, simplicity and low cost; 4) high volume throughput; 5) quantitative measure of receptor occupancy and efficacy; 6) adaptable to hundreds of receptors and subtypes. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述（研究者的摘要）：该提案解决了 NIND的分子神经生物学兴趣来自最新的综合 招标。 高通量，基于荧光的技术 提出了测量受体介导的细胞反应。 目标是 为了开发能够测量细胞内Ca2+的光学探针 由荧光染料报告的大量应用的信号这样 作为筛选许多合成或天然化合物。 许多 现在可以使用记者染料来测量细胞内Ca2+信号 存在以下表面膜受体激活和方法进行测试 如果这些信号通过肌力或代谢机制转导。 光学探针设计将结合有效的光吞吐量， 细胞灌注和快速数据获取。 商业应用 发展的是快速，廉价和定量的测定 将由受体作为合同服务提供 制药公司，政府和大学的生物学 筛选新颖的合成和自然发生的化合物 用于新型的神经活动药物。 主要优势 方法包括：1）独立于放射性同位素； 2）独立 来自实验动物； 3）速度，简单性和低成本； 4）高 音量吞吐量； 5）量化受体占用和 功效; 6）适应数百种受体和亚型。 拟议的商业应用程序：不可用