GENETICS AND FUNCTION OF H-2 CLASS II (IA) GENES

描述(根据申请者的抽象和具体目标进行调整)：此 这项研究将通过产生和分析体内II类结构/功能 分析突变的II类转基因(TG)小鼠品系。具体目标1 建议产生适当的TG小鼠来检查Aβ的作用 抗原提呈、T细胞谱系等方面的突变 自身免疫力。特定目标2将通过以下方式检查第二类与CD4的相互作用 突变Aβ基因推测的CD4结合位点并产生 适当的转基因小鼠。具体目标3将分析涉及的II类站点 转基因L细胞体外和体内TG中二聚体的形成 老鼠。这将通过在据信是 参与二聚体-二聚体的形成。具体目标4将重点放在 产生可溶的II类A和E类分子用于耐受性研究。

DESCRIPTION (Adapted for the Applicant's abstract and Specific Aims.): This study will analyze class II structure/function in vivo by producing and analyzing mutant class II transgenic (tg) mouse strains. Specific Aim 1 proposes to generate appropriate tg mice to examine the role of A beta mutations on antigen presentation, the T-cell repertoire, and on autoimmunity. Specific Aim 2 will examine class II interaction with CD4 by mutating the presumptive CD4 binding site of A beta genes and generating appropriate tg mice. Specific Aim 3 will analyze class II sites involved in dimer-dimer formation in vitro in transfected L cells and in vivo in tg mice. This will be approached by introducing mutations at sites believed to be involved in dimer-dimer formation. Specific Aim 4 will focus on generating soluble class II A and E molecules for tolerance studies.