MOLECULAR TYPING OF RECURRENT CHLAMYDIAL STDS

复发性衣原体标准的分子分型

• 批准号: 2672709
• 负责人: DEBORAH Anne DEAN
• 金额: $ 15.16万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至 2001-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2672709
• 关键词: Chlamydia trachomatis; adolescence (12-20); bacterial genetics; bacterial proteins; chlamydial disease; clinical research; epidemiology; genetic strain; genotype; human subject; membrane proteins; polymerase chain reaction; relapse /recurrence; serotyping; sexually transmitted diseases; sign /symptom

DESCRIPTION (Adapted from the applicant's abstract): Chlamydia trachomatis is the leading cause of sexually transmitted diseases (STD) in the developed world. The majority of these infections are subclinical and non-acute in presentation which accounts for the large number of women with tubal infertility and ectopic pregnancies that are so prevalent today. Further, up to 50% of adolescent and young adult women become reinfected with Chlamydia which contributes to tubal scarring. Thus, adolescents are an important target group in which to analyze transmission patterns and types of recurrence in order to design appropriate interventional strategies. Strain typing is essential for defining the molecular epidemiology of chlamydial infections within the context of different chlamydial asymptomatic and symptomatic disease states that include urethritis, cervicitis, pelvic inflammatory disease, and recurrent infection. Monoclonal antibodies to the major outer membrane protein (MOMP) of C. trachomatis have historically been used for typing. Yet, serotypes do not reflect the same degree of variation found at the gene and protein level of this organism. ompl genotyping has been developed which is based on the MOMP gene (ompl ). It is capable of detecting new subtypes of currently known serotypes and provides a powerful alternative to immunotyping. Genotyping is an appropriate tool for evaluating transmission patterns and recurrent infections, and for use as a biologic measure of behavior in behavioral related studies and for public health surveillance. Specific Aims: 1) define the ompl genotypes and variants among adolescent males and females who differ by force-of-infection and behavioral variables; 2) analyze transmission patterns of ompl genotypes between partners and within teen groups; 3) evaluate types of recurrent infections that occur in adolescents in comparison with first (naive) or previous infections, define types of mutations that occur in ompl over these time points, and evaluate the role of genotype polymorphism, co-pathogens, and immune response in reinfection at cervical and urethral sites; and 4) define molecular characteristics of genotypes that may be associated with clinical and microbiologic data; analyze the sequence-structure/function relationships of MOMP from prototype and variant strains.

描述（改编自申请人的摘要）：沙眼衣原体 是发达国家性传播疾病（STD）的主要原因 世界。 这些感染大多数是亚临床和非急性的 介绍了许多患有输卵管疾病的女性 如今，不孕症和宫外孕非常普遍。 更远， 高达 50% 的青少年和年轻成年女性再次感染 衣原体会导致输卵管疤痕形成。 因此，青少年是一个 分析传播模式和类型的重要目标群体 以便设计合适的干预策略。 毒株分型对于定义病毒的分子流行病学至关重要 不同衣原体背景下的衣原体感染 无症状和有症状的疾病状态，包括尿道炎， 宫颈炎、盆腔炎、反复感染。 C. 主要外膜蛋白 (MOMP) 的单克隆抗体。 沙眼历来被用于打字。 然而，血清型并不 反映了在基因和蛋白质水平上发现的相同程度的变异 这个有机体。 ompl 基因分型的开发基于 MOMP 基因 (ompl)。它能够检测当前的新亚型 已知的血清型，并提供了免疫分型的强大替代方案。 基因分型是评估传播模式和 反复感染，并用作行为的生物学测量 行为相关研究和公共卫生监测。 具体目标：1）定义青少年中的 ompl 基因型和变异 男性和女性的感染力和行为变量有所不同； 2）分析ompl基因型在伴侣之间的传播模式 在青少年群体内； 3）评估反复感染的类型 与首次（幼稚）或以前的感染相比，青少年定义 ompl 在这些时间点发生的突变类型，并评估 基因型多态性、共同病原体和免疫反应在疾病中的作用 宫颈和尿道部位的再感染； 4) 定义分子 可能与临床和临床相关的基因型特征 微生物数据；分析序列-结构/功能关系 来自原型菌株和变异菌株的 MOMP。