CONTROL OF HYPOTHALAMIC TYROSINE HYDROXYLASE

描述(改编自《调查员摘要》)：主要目标 这一应用的目的是评估细胞内机制， PRL、E和P影响TIDA神经元的TH活性。目标1将发表演讲 TH的磷酸化在血管紧张素转换酶条件下的潜在作用 PRL升高。作为对PRL的反应，影响TH的蛋白激酶将 被研究。第二个目标将研究E通过哪些机制 改变胎儿下丘脑细胞培养系统中的TH活性。这个 调查将确定E是否会改变TH的mRNA水平。第三 AIM将集中于P对大鼠脑内TH活性的急性抑制作用 胎儿下丘脑培养细胞，并将解决 磷是否能诱导TH去磷酸化。

DESCRIPTION (Adapted from the Investigator's Abstract): The major goals of this application are to evaluate intracellular mechanisms which permit PRL, E, and P to affect TH activity in TIDA neurons. Aim 1 will address the potential role of phosphorylation of TH during conditions of elevated PRL. Protein kinases which affect TH as a response to PRL will be studied. The second aim will examine the mechanisms by which E alters TH activity in a fetal hypothalamic cell culture system. The investigations will determine whether E alters TH mRNA levels. The third aim will focus on acute inhibitory effects of P on TH activity in the fetal hypothalamic cultured cells, and will address the question of whether P can induce dephosphorylation of TH.