ACTIONS OF ACETYLCHOLINE ON NEOCORTICAL INTERNEURONS
ACTIONS OF ACETYLCHOLINE ON NEOCORTICAL INTERNEURONS
批准号:
2647493
负责人:
ZIXIU XIANG
金额:
$3.02万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1998
资助国家:
美国
项目状态:
未结题
起止时间:
1998-08-01 至
中文摘要
描述
乙酰胆碱(Acetylcholine,ACh)是一种神经递质,具有强大的调节作用,
对哺乳动物CNS中神经元兴奋性的影响。相比
ACh对锥体细胞的兴奋作用已被充分研究,但知之甚少
胆碱能受体激活对大脑皮层的直接影响
中间神经元为了验证这一假设,各种类型的中间神经元,
大鼠视皮层V层对乙酰胆碱反应差异
不同类型的胆碱能受体亚型,全细胞
来自切片中可视化神经元的电流/电压钳记录将被
与细胞内标记、细胞外药物
应用、单细胞PCR和免疫细胞化学。具体而言是
膜电位和输入电阻的胆碱能调节
将检查不同类型的中间神经元。的参与
不同类型或亚型的ACh受体将通过(1)
应用不同的激动剂和/或拮抗剂;(2)单细胞PCR
靶向编码烟碱α 4和β 2亚基的mRNA;和(3)
烟碱亚基的荧光抗体标记。离子
乙酰胆碱的作用机制将通过分析
结合离子浓度的变化的电压钳数据。
在不同条件下,I/A,I/M和AHP电流的M.
还将研究中间神经元。这项研究将提供
皮层抑制功能调节的新信息
和皮层信息处理。结果也将产生影响
用于神经系统疾病,如癫痫,其中皮质去抑制
被认为是一种重要的潜在机制,
疾病,其中功能异常可能导致,至少部分,
失去了皮层胆碱能神经支配
英文摘要
DESCRIPTION
Acetylcholine (ACh) is a neurotransmitter that has powerful modulatory
influences on neuronal excitability in the mammalian CNS. In contrast to
well-studied excitatory actions of ACh on pyramidal cells, little is known
about direct effects of cholinergic receptor activation on neocortical
interneurons. To test the hypothesis that various types of interneurons in
layer V of rat visual cortex respond to ACh differently due to activation
of diverse type of subtypes of cholinergic receptors, whole cell
current/voltage clamp recordings from visualized neurons in slices will be
used in conjunction with intracellular labeling, extracellular drug
application, single-cell PCR, and immunocytochemistry. Specifically, the
cholinergic modulation of a membrane potential and input resistance in
different types of interneurons will be examined. The involvement of
different types or subtypes of ACh receptors will be assessed by (1)
application of different agonists and/or antagonists; (2) single-cell PCR
targeting of mRNAs coding for nicotinic alpha4 and beta2 subunits; and (3)
fluorescent antibody labeling of the nicotinic subunits. The ionic
mechanisms underlying the actions of ACh will be determined by analysis of
voltage clamp data in conjunction with alterations of ionic concentration.
The muscarinic modulation of I/A, I/M and AHP currents in different
interneurons will also be investigated. This proposed study will provide
new information relevant to functional modulation of cortical inhibition
nd cortical information processing. Results will also have implications
for neurological disorders, such as epilepsy, where cortical disinhibition
is hypothesized to be an important underlying mechanism and Alzheimer's
disease, in which functional abnormalities may result, at least in part,
from a loss of cortical cholinergic innervation.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
M1 and M4 mAChRs in striatum-implication in treatment of movement disorders
-
批准号:7889856
-
项目类别:
-
资助金额:$33.91万
-
财政年份:2010
-
负责人:ZIXIU XIANG
-
依托单位:
M1 and M4 mAChRs in striatum-implication in treatment of movement disorders
-
批准号:8247009
-
项目类别:
-
资助金额:$33.44万
-
财政年份:2010
-
负责人:ZIXIU XIANG
-
依托单位:
M1 and M4 mAChRs in striatum-implication in treatment of movement disorders
-
批准号:8450830
-
项目类别:
-
资助金额:$32.27万
-
财政年份:2010
-
负责人:ZIXIU XIANG
-
依托单位:
M1 and M4 mAChRs in striatum-implication in treatment of movement disorders
-
批准号:8049123
-
项目类别:
-
资助金额:$33.39万
-
财政年份:2010
-
负责人:ZIXIU XIANG
-
依托单位: