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ACTIONS OF ACETYLCHOLINE ON NEOCORTICAL INTERNEURONS

ACTIONS OF ACETYLCHOLINE ON NEOCORTICAL INTERNEURONS
乙酰胆碱对新皮质中间神经元的作用
批准号:
2647493
负责人:
ZIXIU XIANG
金额:
$3.02万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1998
资助国家:
美国
项目状态:
未结题
起止时间:
1998-08-01 至

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中文摘要
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英文摘要
DESCRIPTION Acetylcholine (ACh) is a neurotransmitter that has powerful modulatory influences on neuronal excitability in the mammalian CNS. In contrast to well-studied excitatory actions of ACh on pyramidal cells, little is known about direct effects of cholinergic receptor activation on neocortical interneurons. To test the hypothesis that various types of interneurons in layer V of rat visual cortex respond to ACh differently due to activation of diverse type of subtypes of cholinergic receptors, whole cell current/voltage clamp recordings from visualized neurons in slices will be used in conjunction with intracellular labeling, extracellular drug application, single-cell PCR, and immunocytochemistry. Specifically, the cholinergic modulation of a membrane potential and input resistance in different types of interneurons will be examined. The involvement of different types or subtypes of ACh receptors will be assessed by (1) application of different agonists and/or antagonists; (2) single-cell PCR targeting of mRNAs coding for nicotinic alpha4 and beta2 subunits; and (3) fluorescent antibody labeling of the nicotinic subunits. The ionic mechanisms underlying the actions of ACh will be determined by analysis of voltage clamp data in conjunction with alterations of ionic concentration. The muscarinic modulation of I/A, I/M and AHP currents in different interneurons will also be investigated. This proposed study will provide new information relevant to functional modulation of cortical inhibition nd cortical information processing. Results will also have implications for neurological disorders, such as epilepsy, where cortical disinhibition is hypothesized to be an important underlying mechanism and Alzheimer's disease, in which functional abnormalities may result, at least in part, from a loss of cortical cholinergic innervation.
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  • 项目类别:
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  • 财政年份:
    2010
  • 负责人:
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  • 项目类别:
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  • 项目类别:
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    8049123
  • 项目类别:
  • 资助金额:
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  • 财政年份:
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