EXCITATORY TRANSMISSION IN TEMPORAL LOBE EPILEPSY

理解苔藓纤维发芽在植物生长发育中的作用 癫痫患者 患者在开发这种疾病的新疗法方面起着关键作用。 以前的研究利用了低分辨率的细胞外和 细胞内记录研究苔藓的功能作用 纤维 发芽了。本提案概述了使用HIGH 决议 兴奋性突触后全细胞电压钳记录 洋流 电刺激和电刺激对颗粒细胞(EPSCs)的影响 光刺激。功能性兴奋的发生率和程度 颗粒细胞之间的突触将在正常情况下进行研究 冬虫夏草制剂中苔藓纤维发芽条件及后续研究 难度和复杂性增加：1)红藻氨酸处理 器官型 海马片培养；分离急性海马片 从2) 匹罗卡品治疗的大鼠；3)人癫痫海马区。这个 复发性兴奋性突触连接的特性将是 考查 通过确定介导的复发EPSCs的比例 由NMDA提供 和非NMDA谷氨酸受体。γ-氨基丁酸能对人体的影响 传输 对复发的EPSCs也将进行调查。这些实验 是 旨在确定颗粒细胞是否具有功能 复发性 苔藓纤维后与其他颗粒细胞的兴奋性突触 萌发和定义这些循环的性质 兴奋的 突触连接。

Understanding the functional role of mossy fiber sprouting in epileptic patients is pivotal in developing new therapies for this disease. Previous studies have utilized low resolution extracellular and intracellular recording to investigate the functional role of mossy fiber sprouting. This proposal outlines a strategy to use high resolution whole cell voltage clamp recording of excitatory postsynaptic currents (EPSCs) in granule cells evoked with both electrical and photostimulation. The incidence and extent of functional excitatory synapses between granule cells will be investigated under normal conditions and following mossy fiber sprouting in preparations of increasing difficulty and complexity: 1) Kainic acid treated organotypic hippocampal slice cultures; and acute hippocampal slices isolated from 2) Pilocarpine treated rats; and 3) Human epileptic hippocampus. The properties of recurrent excitatory synaptic connections will be examined by determining the proportion of recurrent EPSCs that is mediated by NMDA and nonNMDA glutamate receptors. The effects of GABAergic transmission on recurrent EPSCs also will be investigated. These experiments are designed to determine whether granule cells from functional recurrent excitatory synapses with other granule cells following mossy fiber sprouting and to define the properties of these recurrent excitatory synaptic connections.