MORPHOLOGICAL DYNAMICS OF LIVING NEURONS IN SLICES

这项研究探索了一种新的器官类型培养系统的潜力。 作为体内神经移植的模型，以及作为研究 突触可塑性的形态相关性。我会研究一下 胚胎大鼠海马神经元移植培养的发育 新生大鼠海马区脑片。我会观察这个过程 利用延时双光子，在发生时移植集成 显微镜。我会观察切片年龄和移植的效果 移植的CA1和CA3锥体突起的定位 神经元。我将量化细胞的动态形态参数 移植细胞，如轴突生长和回缩率，分支 与寄主的形成和细胞体迁移相比较 细胞。消极剂将在时间流逝期间应用于切片 成像以查看切片中自发活动的总体水平 影响神经形态。这些发现将对那些 对学习和记忆的基础感兴趣，以及 海马区移植改善神经退行性变的作用 疾病，如阿尔茨海默氏症。

This study explores the potentials of a new organotypic culture system as a model for in vivo neural transplants, and as a tool for studying the morphological correlates of synaptic plasticity. I will study the development of embryonic rat hippocampal neurons transplanted to cultured hippocampal slices from neonatal rats. I will observe the process of transplant integration while it happens, using time-lapse 2-photon microscopy. I will observe the effects of slice age and transplant location on the neurite outgrowth of transplanted CA1 and CA3 pyramidal neurons. I will quantify dynamic morphological parameters of the transplanted cells, such as neurite growth and retraction rates, branch formation, and cell body migration, in comparison to those of the host cells. Depolarizing agents will be applied to slices during timelapse imaging to see how the overall level of spontaneous activity in the slice influences neural morphology. The findings will be useful to those interested in the bases of learning and memory, and the potential for hippocampal transplants to ameliorate the effects of neurodegenerative disorders, such as Alzheimer's disease.