DOC-1 MEDIATED APOPTOSIS IN MAMMALIAN CELLS

DOC-1 介导的哺乳动物细胞凋亡

• 批准号: 6270242
• 负责人: John R. Basile
• 金额: $ 5.72万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6270242
• 关键词: DOC; MEDIATED; APOPTOSIS; MAMMALIAN; CELLS

DOC-l Mediated Apoptosis in Mammalian Cells I am currently doing a rotation in the laboratory of Dr. David Wong attempting to characterize the function and mechanisms of action of the tumor suppressor gene human doc-l. This gene was originally discovered by Dr. Wong's group to be missing in certain types of oral squamous cell carcinomas. It is suspected to induce apoptosis when activated and lead to uncontrolled cell growth when deleted. I have cloned the doc-l gene into an inducible gene expression system. This allows for activation of transcription of doc-l when administering the inducing agent, which is a steroid hormone, but prevents transcription when not induced. This first step was an important prerequisite for future cell culture because cells containing an intact and actively transcribed doc-l gene are difficult to grow and maintain. The second phase of my research involves transfecting this vector construct into transformed mammalian cells, inducing the gene with the hormone and observing the effects in vitro. I eventually would like to establish clones of cells with the inducible form of doc-l stably integrated into the genome of several types of transformed mammalian cell lines.

DOC-1介导的哺乳动物细胞凋亡 我目前在大卫博士的实验室做一个轮换 Wong试图描述的功能和机制 肿瘤抑制基因人doc-1的作用。 该基因 黄博士的小组最初发现在某些 口腔鳞状细胞癌的类型 它被怀疑是诱导 细胞凋亡，并导致不受控制的细胞生长时， 的页面不存在或 我已经将doc-1基因克隆到一个诱导型基因表达载体中 系统 这允许在以下情况下激活doc-l的转录： 施用诱导剂，其是类固醇激素，但是 在未被诱导时阻止转录。 这第一步是一个 这是未来细胞培养的重要先决条件， 含有完整的和活跃转录的DOC-1基因， 难以生长和维持。 我研究的第二阶段涉及转染该载体 构建体转化到转化的哺乳动物细胞中， 并在体外观察其作用。 我最终 想要建立具有可诱导形式的细胞克隆， DOC-1稳定整合到几种类型的 转化的哺乳动物细胞系。