NUCLEOTIDE INDUCED STRUCTURAL CHANGES IN MYOSINS

描述：(摘自摘要)本研究的长期目标 都是为了可视化肌球蛋白头部的构象变化 连接和分离的横桥循环阶段，并将这些 在Mosin机械力化学背景下的观察。在 这里描述的实验，冷冻电子显微镜和图像分析将 用于回答以下基本问题：1)是 ADP诱导的肌球蛋白和刷状缘肌球蛋白Ia的变化 肌球蛋白的总体特征？2)是否还有其他戏剧性的 与周期早期的附加状态相关的构象变化？ 3)肌球蛋白头部在肌动蛋白附着之前的构象 有别于ADP.PI、ADP和Rigor附着态？数据将被 获取旨在建立与生物化学有关的一般原则 肌球蛋白马达和Pave的转变、构象变化和功能 更全面地了解肌球蛋白机械力化学的途径。

DESCRIPTION: (Adapted from abstract) The long term goals of this research are to visualize the conformational changes in the myosin head in both the attached and detached stages of the crossbridge cycle, and to place these observations within the context of mosin mechanochemistry. In the experiments described here, cryo-electron microscopy and image analysis will be used to answer the following fundamental questions: 1)Are the ADP-induced changes seen in smooth muscle myosin and brush border myosin I a charactistic of myosins in general? 2) Are there additional dramatic conformational changes associated with attached states earlier in the cycle? And 3) Is the conformation of the myosin head prior to actin attachment distinct from the ADP.Pi, ADP and rigor attached states? The data to be obtain is designed to establish general principles relating biochemical transitions, conformational changes and function in myosin motors, and pave the way for a more complete understanding of myosin mechanochemistry.