NUCLEOTIDE INDUCED STRUCTURAL CHANGES IN MYOSINS

核苷酸诱导的肌球蛋白结构变化

• 批准号: 2683352
• 负责人: RONALD A MILLIGAN
• 金额: $ 36.82万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-19 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2683352
• 关键词: actins; adenosine diphosphate; biochemistry; biomechanics; brush border membrane; conformation; electron microscopy; freeze etching; guanosine diphosphate; muscle function; myosins; smooth muscle

DESCRIPTION: (Adapted from abstract) The long term goals of this research are to visualize the conformational changes in the myosin head in both the attached and detached stages of the crossbridge cycle, and to place these observations within the context of mosin mechanochemistry. In the experiments described here, cryo-electron microscopy and image analysis will be used to answer the following fundamental questions: 1)Are the ADP-induced changes seen in smooth muscle myosin and brush border myosin I a charactistic of myosins in general? 2) Are there additional dramatic conformational changes associated with attached states earlier in the cycle? And 3) Is the conformation of the myosin head prior to actin attachment distinct from the ADP.Pi, ADP and rigor attached states? The data to be obtain is designed to establish general principles relating biochemical transitions, conformational changes and function in myosin motors, and pave the way for a more complete understanding of myosin mechanochemistry.

描述：（改编自摘要）本研究的长期目标 是为了可视化肌球蛋白头部的构象变化， 十字桥循环的连接和分离阶段，并将这些 在mosin机械化学的背景下观察。 在 这里描述的实验，冷冻电子显微镜和图像分析将 可以用来回答以下基本问题：1） ADP诱导的平滑肌肌球蛋白和刷状缘肌球蛋白I a的变化 肌球蛋白的特征 2)是否有额外的戏剧性 与周期早期附着状态相关的构象变化？ 3）肌球蛋白头部的构象是否先于肌动蛋白附着 与ADP不同。π，ADP和严格连接状态？ 的数据 获得旨在建立有关生物化学的一般原则， 肌球蛋白马达的转变、构象变化和功能， 更全面地了解肌球蛋白机械化学的途径。