IMMUNODOMINANT STRESS PROTEINS OF P GINGIVALIS

微生物在感染过程中产生新的蛋白质。 其中许多蛋白质的表达代表了对应激的反应 由宿主施加的(应激蛋白，HSP)，对 微生物追踪感染过程的能力。为被告辩护 宿主通常依赖于对这些蛋白的免疫识别。 本申请中提供的初步数据显示，体液免疫 其中一种名为HSP90的蛋白质与牙龈健康和 牙龈卟啉单胞菌的低水平定植 牙周病原体。关于应激蛋白的数据很少。 牙周病原体。应激蛋白是免疫优势蛋白， 其他非口腔细菌感染中的重要抗原。调查 牙周病原体的应激蛋白可能对我们的 了解牙周病的病因学。 这个实验室的长期目标是确定压力(S)的作用 通过研究它们在牙周病过程中的作用 表达、调控和与寄主防御机制的相互作用。这个 正如本提案中所概述的，第一步是确定90KD的特征 牙龈假单胞菌的应激蛋白，根据我们的初步研究， 在生物体在牙周疾病中的作用中起着重要作用。具体的 本方案中要检验的假设是：HSP90由P. 在模拟感染过程的条件下，牙周炎。快递 牙周炎假单胞菌对HSP90的毒力和抗体 针对牙龈假单胞菌，HSP90具有保护性作用。我们打算测试一下 这一假设是通过：(1)刻画了P. (2)克隆和测序。 牙龈热休克蛋白90基因；(3)进行免疫电子显微镜研究 将HSP90蛋白定位于牙龈假单胞菌表面和内部 (4)评价HSP90对P. 在两种动物模型中发现了牙周炎。

Microorganisms produce new proteins during the infectious process. Expression of many of these proteins represents the response to stresses imposed by the host (stress-proteins, HSP) and is important to the microorganism's ability to pursue the infectious process. Defense of the host is often dependent on immunologic recognition of these proteins. Preliminary data presented in this application shows that humoral immunity to one such protein, HSP90, is associated with both gingival health and low level colonization by Porphyromonas gingivalis, one of the principal periodontal pathogens. There is a paucity of data on the stress proteins of the periodontal pathogens. The stress proteins are immunodominant and important antigens in other non-oral bacterial infections. Investigations of the stress proteins of periodontal pathogens may be crucial to our understanding of the etiology of periodontal disease. The long-term goals of this laboratory are to define the role(s) of stress proteins in the periodontal disease process by investigating their expression, regulation and interaction with host defense mechanisms. The first step, as outlined in this proposal, is to characterize the 90 kD stress protein of P. gingivalis which, based on our preliminary studies, is important in the organism's role in periodontal disease. The specific hypothesis to be tested in this proposal is: HSP90 is expressed by P. gingivalis during conditions that mimic the infectious process. Express of HSP90 by P. gingivalis contributes to its virulence and antibodies directed against P. gingivalis HSP90 are protective. We propose to test this hypothesis by: (1) characterizing the conditions under which P. gingivalis HSP90 is expressed; (2) cloning and sequencing the P. gingivalis HSP90 gene; (3) performing immuno electronmicroscopic studies to localize the HSP90 protein on the surface and inside the P. gingivalis cell; (4) evaluating the contribution HSP90 to the virulence of P. gingivalis in two animal models.