YEAST GENES INVOLVED IN GENERAL TRANSCRIPTIONAL CONTROL

参与一般转录控制的酵母基因

基本信息

  • 批准号:
    2770958
  • 负责人:
  • 金额:
    $ 32.79万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1990
  • 资助国家:
    美国
  • 起止时间:
    1990-09-01 至 1999-09-09
  • 项目状态:
    已结题

项目摘要

The goal of this research is to elucidate the mechanisms of eucaryotic gene regulation. The system chosen for study is the yeast regulatory complex consisting of CCR4, CAF1, CAF185 and other proteins which are required for the proper expression of a number of diverse genes in yeast. At least five proteins, including CAF1 and CAF185, have been identified as co-immuneprecipitating with CCR4 and another seven appear to co-purify with it. Homologs to CAF1 have been identified in higher organisms, and mammalian CAF1 can substitute for several of yeast CAF1 functions. Biochemical evidence indicates that CCR4 and CAF1 can be specifically retained on a RNA polymerase H C-terminal domain (CTD) affinity column, suggesting that CCR4/CAF1 may be peripheral components of the core transcription apparatus. The CCR4 protein complex is distinct from several other regulatory complexes that have been identified in yeast including that of the SNF/SWI proteins, the holoenzyme SRB complex, the TATA-binding protein associated factors, and the SPT6 complex. The central focus of this grant proposal is two-fold: 1) Identify and characterize the components of the CCR4 protein complex and 2) determine how this complex interacts with the transcriptional machinery to affect transcription. The components of the CCR4 complex will be identified by purifying the complex to homogeneity. The genes for individual components will be cloned following microsequencing of peptide fragments from each protein. The assembly and protein interactions within the complex will be analyzed by genetic and biochemical techniques. The function of CCR4 and its associated proteins will be elaborated by determining their effects on in vitro transcription, by identifying their contacts with the transcriptional machinery, and by examining their link to the CTD of RNA polymerase II. The relationship of CCR4 to that of DBF2, a late mitotic cell-cycle regulated protein kinase that associates genetically with CCR4, will also be studied. Finally, additional regulatory proteins that functionally relate with CCR4 will be identified by characterizing and cloning the genes which when mutated result in synthetic lethality in combination with a ccr4 deletion.
本研究的目的是阐明真核生物的机制

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

CLYDE L DENIS其他文献

CLYDE L DENIS的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('CLYDE L DENIS', 18)}}的其他基金

Characterization of the mRNP closed-loop structure
mRNP 闭环结构的表征
  • 批准号:
    8722662
  • 财政年份:
    2013
  • 资助金额:
    $ 32.79万
  • 项目类别:
Characterization of the mRNP closed-loop structure
mRNP 闭环结构的表征
  • 批准号:
    8496294
  • 财政年份:
    2013
  • 资助金额:
    $ 32.79万
  • 项目类别:
Characterization of the mRNP closed-loop structure
mRNP 闭环结构的表征
  • 批准号:
    9333556
  • 财政年份:
    2013
  • 资助金额:
    $ 32.79万
  • 项目类别:
Factors affecting mRNA deadenylation
影响 mRNA 去腺苷化的因素
  • 批准号:
    8005169
  • 财政年份:
    2010
  • 资助金额:
    $ 32.79万
  • 项目类别:
Yeast PUF3 control of mRNA expression
酵母 PUF3 控制 mRNA 表达
  • 批准号:
    7850034
  • 财政年份:
    2006
  • 资助金额:
    $ 32.79万
  • 项目类别:
Yeast PUF3 control of mRNA expression
酵母 PUF3 控制 mRNA 表达
  • 批准号:
    7128339
  • 财政年份:
    2006
  • 资助金额:
    $ 32.79万
  • 项目类别:
YEAST GENES INVOLVED IN GENERAL TRANSCRIPTIONAL CONTROL
参与一般转录控制的酵母基因
  • 批准号:
    6385887
  • 财政年份:
    1990
  • 资助金额:
    $ 32.79万
  • 项目类别:
YEAST GENES INVOLVED IN GENERAL TRANSCRIPTIONAL CONTROL
参与一般转录控制的酵母基因
  • 批准号:
    6180117
  • 财政年份:
    1990
  • 资助金额:
    $ 32.79万
  • 项目类别:
YEAST GENES INVOLVED IN GENERAL TRANSCRIPTIONAL CONTROL
参与一般转录控制的酵母基因
  • 批准号:
    2907736
  • 财政年份:
    1990
  • 资助金额:
    $ 32.79万
  • 项目类别:
YEAST GENES INVOLVED IN GENERAL TRANSCRIPTIONAL CONTROL
参与一般转录控制的酵母基因
  • 批准号:
    2180718
  • 财政年份:
    1990
  • 资助金额:
    $ 32.79万
  • 项目类别:

相似海外基金

Cellular membrane affinity chromatography kit for drug discovery
用于药物发现的细胞膜亲和层析试剂盒
  • 批准号:
    10506915
  • 财政年份:
    2021
  • 资助金额:
    $ 32.79万
  • 项目类别:
Cellular membrane affinity chromatography kit for drug discovery
用于药物发现的细胞膜亲和层析试剂盒
  • 批准号:
    10325006
  • 财政年份:
    2021
  • 资助金额:
    $ 32.79万
  • 项目类别:
SBIR Phase I: A New Class of Immobilized Metal Affinity Chromatography Resins
SBIR 第一阶段:一类新型固定金属亲和色谱树脂
  • 批准号:
    1746198
  • 财政年份:
    2018
  • 资助金额:
    $ 32.79万
  • 项目类别:
    Standard Grant
Marine speciation of nickel using immobilized nickel affinity chromatography
使用固定镍亲和色谱法测定镍的海洋形态
  • 批准号:
    512537-2017
  • 财政年份:
    2017
  • 资助金额:
    $ 32.79万
  • 项目类别:
    University Undergraduate Student Research Awards
I-Corps: Commercialization of Immobilized Metal Affinity Chromatography Resins Based on Nanomaterials
I-Corps:基于纳米材料的固定化金属亲和层析树脂的商业化
  • 批准号:
    1404605
  • 财政年份:
    2014
  • 资助金额:
    $ 32.79万
  • 项目类别:
    Standard Grant
Antibody Purification via Affinity Chromatography that Utilizes the Unconventional Nucleotide Binding Site
利用非常规核苷酸结合位点通过亲和色谱法纯化抗体
  • 批准号:
    1263713
  • 财政年份:
    2013
  • 资助金额:
    $ 32.79万
  • 项目类别:
    Continuing Grant
Development of multivalent DNA network based affinity chromatography diagnostics for isolating circulating tumour cells
开发基于多价 DNA 网络的亲和色谱诊断法,用于分离循环肿瘤细胞
  • 批准号:
    425749-2012
  • 财政年份:
    2012
  • 资助金额:
    $ 32.79万
  • 项目类别:
    Postgraduate Scholarships - Master's
Next-Generation Affinity Chromatography with PEGylated Ligands
使用聚乙二醇化配体的新一代亲和色谱法
  • 批准号:
    1159886
  • 财政年份:
    2012
  • 资助金额:
    $ 32.79万
  • 项目类别:
    Standard Grant
Immobilized zirconium ion affinity chromatography for specific enrichment of phosphoproteins
用于磷蛋白特异性富集的固定化锆离子亲和层析
  • 批准号:
    19560760
  • 财政年份:
    2007
  • 资助金额:
    $ 32.79万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Accelerating drug discovery using frontal affinity chromatography/mass spectrometry
使用正面亲和色谱/质谱加速药物发现
  • 批准号:
    234753-2000
  • 财政年份:
    2003
  • 资助金额:
    $ 32.79万
  • 项目类别:
    Collaborative Research and Development Grants
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了