YEAST GENES INVOLVED IN GENERAL TRANSCRIPTIONAL CONTROL
参与一般转录控制的酵母基因
基本信息
- 批准号:2770958
- 负责人:
- 金额:$ 32.79万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1990
- 资助国家:美国
- 起止时间:1990-09-01 至 1999-09-09
- 项目状态:已结题
- 来源:
- 关键词:DNA directed RNA polymerase affinity chromatography alcohol dehydrogenase cell cycle proteins cell growth regulation chemical binding chimeric proteins fungal genetics gel filtration chromatography gene deletion mutation genetic transcription immunoprecipitation intermolecular interaction molecular cloning molecular size northern blottings phosphorylation protein kinase protein purification protein reconstitution protein sequence protein structure function suppressor mutations transcription factor western blottings
项目摘要
The goal of this research is to elucidate the mechanisms of eucaryotic
gene regulation. The system chosen for study is the yeast regulatory
complex consisting of CCR4, CAF1, CAF185 and other proteins which are
required for the proper expression of a number of diverse genes in
yeast. At least five proteins, including CAF1 and CAF185, have been
identified as co-immuneprecipitating with CCR4 and another seven appear
to co-purify with it. Homologs to CAF1 have been identified in higher
organisms, and mammalian CAF1 can substitute for several of yeast CAF1
functions. Biochemical evidence indicates that CCR4 and CAF1 can be
specifically retained on a RNA polymerase H C-terminal domain (CTD)
affinity column, suggesting that CCR4/CAF1 may be peripheral components
of the core transcription apparatus. The CCR4 protein complex is
distinct from several other regulatory complexes that have been
identified in yeast including that of the SNF/SWI proteins, the
holoenzyme SRB complex, the TATA-binding protein associated factors, and
the SPT6 complex. The central focus of this grant proposal is two-fold:
1) Identify and characterize the components of the CCR4 protein complex
and 2) determine how this complex interacts with the transcriptional
machinery to affect transcription.
The components of the CCR4 complex will be identified by purifying the
complex to homogeneity. The genes for individual components will be
cloned following microsequencing of peptide fragments from each protein.
The assembly and protein interactions within the complex will be
analyzed by genetic and biochemical techniques. The function of CCR4 and
its associated proteins will be elaborated by determining their effects
on in vitro transcription, by identifying their contacts with the
transcriptional machinery, and by examining their link to the CTD of RNA
polymerase II. The relationship of CCR4 to that of DBF2, a late mitotic
cell-cycle regulated protein kinase that associates genetically with
CCR4, will also be studied. Finally, additional regulatory proteins that
functionally relate with CCR4 will be identified by characterizing and
cloning the genes which when mutated result in synthetic lethality in
combination with a ccr4 deletion.
本研究的目的是阐明真核生物的机制
项目成果
期刊论文数量(0)
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专利数量(0)
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{{ truncateString('CLYDE L DENIS', 18)}}的其他基金
Characterization of the mRNP closed-loop structure
mRNP 闭环结构的表征
- 批准号:
8722662 - 财政年份:2013
- 资助金额:
$ 32.79万 - 项目类别:
Characterization of the mRNP closed-loop structure
mRNP 闭环结构的表征
- 批准号:
8496294 - 财政年份:2013
- 资助金额:
$ 32.79万 - 项目类别:
Characterization of the mRNP closed-loop structure
mRNP 闭环结构的表征
- 批准号:
9333556 - 财政年份:2013
- 资助金额:
$ 32.79万 - 项目类别:
YEAST GENES INVOLVED IN GENERAL TRANSCRIPTIONAL CONTROL
参与一般转录控制的酵母基因
- 批准号:
6385887 - 财政年份:1990
- 资助金额:
$ 32.79万 - 项目类别:
YEAST GENES INVOLVED IN GENERAL TRANSCRIPTIONAL CONTROL
参与一般转录控制的酵母基因
- 批准号:
6180117 - 财政年份:1990
- 资助金额:
$ 32.79万 - 项目类别:
YEAST GENES INVOLVED IN GENERAL TRANSCRIPTIONAL CONTROL
参与一般转录控制的酵母基因
- 批准号:
2907736 - 财政年份:1990
- 资助金额:
$ 32.79万 - 项目类别:
YEAST GENES INVOLVED IN GENERAL TRANSCRIPTIONAL CONTROL
参与一般转录控制的酵母基因
- 批准号:
2180718 - 财政年份:1990
- 资助金额:
$ 32.79万 - 项目类别:
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