APOPTOSIS IN DROSOPHILA--FROM REAPER TO DEATH

描述：PI将继续研究细胞凋亡的分子控制 在果蝇身上。圆周率显示，收割者基因有一个中心 在启动凋亡中的作用，将以剂量敏感的方式使用 对果蝇的其他细胞死亡基因进行基因筛查。在……里面 初步工作，染色体缺失和点突变已经被 识别哪些增强或抑制死神来源的细胞死亡。在 拟议工作：(1)将延长此类屏幕，以努力收集 效应器基因。它们应该对响应收割机的函数进行编码 表达并负责提供细胞死亡的特定元素 机械设备。(2)效应器基因将在 分子水平。(3)效应器功能丧失突变的表型 将独立于收割机的调制来检查轨迹，以更好地 了解每个基因在控制细胞死亡中的作用。

DESCRIPTION: The PI will continue to study molecular controls of apoptosis in Drosophila. The reaper gene, which was shown by the PI to have a central role in initiation of apoptosis, will be used in a dosage sensitive manner to conduct genetic screens for other cell death genes in Drosophila. In preliminary work, chromosomal deletions and point mutations have been recognized which enhance or suppress reaper derived cell death. In the proposed work: (1) Such screens will be extended in an effort to collect effector genes. These should encode functions responding to reaper expression and responsible for providing specific elements of the cell death machinery. (2) Effector genes will be cloned and characterized at the molecular level. (3) Phenotypes of loss of function mutations at effector loci will be examined independent of modulation of reaper to better understand the role of each gene in control of cell death.