HERITAGE--GENETICS, RESPONSE TO EXERCISE, RISK FACTORS

遗产家族研究的目的是记录 对耐力运动的心血管和代谢反应，以及 遗传因素对伴随性反应的贡献 心血管疾病(CVD)和糖尿病(NIDDM)危险因素。一个 由5所大学组成的联盟正在进行这项研究。第一阶段(1992- 1997年)，大约765名久坐的受试者接受了 同样的计划，为期20周。最终的主文件中会有 来自98个高加索后裔家庭的约495人，两者都有 父母和亲生成年后代以及102人中的270人 黑人家庭。训练前后最大摄氧量和最大摄氧量 次极量和最大量的心血管和代谢反应 对运动进行测量。血脂、脂蛋白、载脂蛋白 以及与脂肪代谢、胰岛素和葡萄糖反应相关的酶 静脉葡萄糖负荷，血浆性类固醇和糖皮质激素， 休息和锻炼血压、体脂、局部脂肪 测定内脏脂肪的分布和分布。在建议的阶段 2、一系列非遗传学研究将在 传承族数据集。生理、行为和社交 的极大和次极大指标的行列式 安静状态下的心肺耐力及其反应 将对培训进行调查，同时考虑到 年龄、性别和种族。将对心血管疾病进行类似的分析 以及研究中监测的NIDDM危险因素。基因分析将 确定遗传力水平，并测试父系或母系 将使用的效应、主基因效应和分离模式 发展关于反应的遗传基础的假设 耐力锻炼。一组候选基因将被分型并 用于关联和连锁研究。差异显示分析 的骨骼肌转录本将用于识别新的候选者 对耐力运动的反应基因。最后，一个全基因组 将进行搜索以定位候选基因组区域和 心肺功能反应的位置候选基因 耐力与CVD和NIDDM危险因素表型。这一阶段 对遗产家族学的研究应提高我们对 常见心脑血管疾病的黑白区别 静坐状态下的NIDDM危险因素及其遗传学研究 这些危险因素对常规耐力运动的适应 强调潜在的种族差异。此应用程序来自 明尼苏达大学临床中心。

The aim of the HERITAGE Family Study is to document cardiovascular and metabolic responses to endurance exercise, and the contribution of genetic factors to the concomitant responses of cardiovascular disease (CVD) and diabetes (NIDDM) risk factors. A consortium of 5 universities is conducting the study. In Phase I (1992- 1997), about 765 sedentary subjects were exercise-trained with the same program for 20 weeks. There will be in the final master file about 495 individuals from 98 families of Caucasian descent with both parents and biological adult offspring and 270 individuals from 102 Black families. Before and after training, maximal oxygen uptake and cardiovascular and metabolic responses to submaximal and maximal exercise were measured. Plasma lipids, lipoproteins, apolipoproteins and enzymes related to lipid-metabolism, insulin and glucose response to an intravenous glucose load, plasma sex steroids and glucocorticoids, resting and exercise blood pressures, and body fat, regional fat distribution and visceral fat were assessed. During the proposed Phase 2, a series of nongenetic studies will be undertaken on the HERITAGE family dataset. Physiolocial, behavioral and social determinants of maximal and submaximal indicators of cardiorespiratory endurance in the sedentary state an din the response to training will be investigated, taking into account the contributions of age, gender and race. Similar analyses will be carried out on the CVD and NIDDM risk factors monitored in the study. Genetic analyses will determine the heritability levels, and test for paternal or maternal effects, major gene effects and segregation patterns which will be used to develop hypotheses concerning genetic bases of the response to endurance exercise. A panel of candidate genes will be typed and used for association and linkage studies. Differential display analyses of skeletal muscle transcripts will be used to identify new candidate genes for the response to endurance exercise. Finally, a genome wide search will be undertaken to locate candidate genomic regions and positional candidate genes for the response of cardiorespiratory endurance and CVD and NIDDM risk factor phenotypes. This phase of the HERITAGE Family Study should increase our understanding of the differences between Black and White for common CVD and NIDDM risk factors in the sedentary state and of the genetics of adaptation to regular endurance exercise in these risk factors with an emphasis on potential racial differences. This application is from the University of Minnesota Clinical Center.