DENTIN MATRIX PROTEIN 1 EXPRESSION IN ODONTOBLAST CELL

这一修订提案的目的是对 牙本质基质蛋白-1（dentinmatrixprotein-1，DNT-1）基因的表达。 1、 一种主要存在于牙本质中的分泌蛋白，但β 1 -1 mRNA 也可通过成骨细胞中的原位杂交检测。 的假设 组织特异性是由于 转录因子和基因DNA序列的研究 上游地区。 具体目标是（1）表征和 比较人和鼠的EST-I基因的DNA序列;（2） 确定赋予组织特异性表达的上游区域， 将表达载体的基因转移到培养的成牙本质细胞和其他细胞中 细胞类型;和（3）确定甲基化在组织中的重要性 具体表达。

This revised proposal is designed to characterize the regulation of expression of the dentin matrix protein - 1 (DMP-1) gene. DMP-1 is a secreted protein found predominately in dentin, but DMP-1 mRNA is also detected by in situ hybridization in osteoblasts. The hypothesis to be tested is that tissue specificity is due to interactions between transcription factors and gene DNA sequences in the DMP-1 promoter and upstream regions. The specific aims are to (1) characterize and compare DNA sequences for the human and murine DMP-1 genes; (2) determine upstream regions that confer tissue-specific expression using gene transfer of expression vectors into cultured odontoblasts and other cell types; and (3) to determine the importance of methylation in tissue specific expression.