DENTIN MATRIX PROTEIN 1 EXPRESSION IN ODONTOBLAST CELL

这项修订后的提案旨在说明监管的特点 牙本质基质蛋白-1基因的表达。DMP-1是 一种分泌型蛋白，主要存在于牙本质中，但DMP-1mRNA 在成骨细胞中也可以通过原位杂交检测到。假说 有待测试的是，组织特异性是由于 DMP-1启动子中的转录因子和基因DNA序列 以及上游地区。具体目标是(1)描述和描述 比较人和小鼠DMP-1基因的DNA序列；(2) 确定赋予组织特异性表达的上游区域 表达载体对成牙本质细胞等的基因转移 细胞类型；以及(3)确定甲基化在组织中的重要性 特定的表达。

This revised proposal is designed to characterize the regulation of expression of the dentin matrix protein - 1 (DMP-1) gene. DMP-1 is a secreted protein found predominately in dentin, but DMP-1 mRNA is also detected by in situ hybridization in osteoblasts. The hypothesis to be tested is that tissue specificity is due to interactions between transcription factors and gene DNA sequences in the DMP-1 promoter and upstream regions. The specific aims are to (1) characterize and compare DNA sequences for the human and murine DMP-1 genes; (2) determine upstream regions that confer tissue-specific expression using gene transfer of expression vectors into cultured odontoblasts and other cell types; and (3) to determine the importance of methylation in tissue specific expression.