NEUROENDOCRINOLOGY OF REPRODUCTIVE AGING

生殖衰老的神经内分泌学

• 批准号: 2667610
• 负责人: PULIYUR S MOHANKUMAR
• 金额: $ 11.52万
• 依托单位: KANSAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-15 至 2000-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2667610
• 关键词: aging; animal old age; catecholamines; dopamine; electrochemistry; estradiol; estrus; gamma aminobutyrate; high performance liquid chromatography; indoleamine; intermolecular interaction; juvenile animal; laboratory rat; luteinizing hormone; neuroendocrine system; neurotransmitter transport; norepinephrine; peptide hormone metabolism; preoptic areas; progesterone; prolactin; radioimmunoassay; secretion; serotonin

The applications long-term objective is to investigate neurodocrine changes responses for age-related loss of reproduction. The specific aims of this application are to use the techniques of high performance liquid chromatography with electrochemical detection and radioimmunoassay to determine age related changes in the release of catecholamines, serotonin, and luteinizing hormone and prolactin. The data on the release of neurotransmitters will be correlated with changes in serum levels of luteinizing hormone and prolactin. Changes in these parameters will also be determined in old acyclic animals. Role of gamma amino butyric acid in aged-related changes in luteinizing in old acyclic animals. Role of gama amino butyric acid in age-related changes in luteinizing hormone secretion and its interaction with norepinephrine will be test in young and aging animals. Changes in the response of neurotransmitters to prolonged exposure to estradiol will be determined by monitoring neurotransmitter release in young cycling animals. Similarly, the involvement of catecholamines in progesterone-induced reinitiation of estrous cycles in middle -aged persistent estrus animals will be examined. Patterns of neurotransmitters and hormone release will also be determined in old animals after induction of estrous cyclicity. The data collected will explain the involvement of neurotransmitters in normal reproductive cycles and in-age related changes in cyclicity. These data will be new and will add to our knowledge of the neuroendocrine involvement in loss of reproductive cyclicity in aging.

应用的长期目标是研究神经内分泌的变化 与年龄相关的生殖能力丧失的反应。 本申请的具体目的是使用高技术 高效液相色谱电化学检测 放射免疫测定，以确定年龄相关的变化，释放 儿茶酚胺、血清素、促黄体激素和催乳素。 数据 神经递质的释放将与 血清促黄体生成素和催乳素水平。 未来基本假设条件发生变化 参数也将在年老的非周期性动物中确定。 伽马的作用 氨基丁酸在老年非周期性黄体生成中的年龄相关变化 动物 γ-氨基丁酸在老年性痴呆中的作用 促黄体激素的分泌及其与去甲肾上腺素的相互作用将 在年轻和年老的动物中进行测试。 答复的变化 神经递质对长期暴露于雌二醇的影响将通过 监测年轻骑自行车动物的神经递质释放。 与此类似， 儿茶酚胺参与甾体酮诱导的 将检查中年持续发情动物的发情周期。 神经递质和激素释放的模式也将被确定 在诱导发情周期后的老年动物中。 收集的数据将解释神经递质参与 正常的生殖周期和与年龄相关的周期性变化。 这些 数据将是新的，并将增加我们的知识，神经内分泌 参与衰老中生殖周期的丧失。