MOLECULAR MECHANISM OF BUTTERFLY WING EYESPOT FORMATION

蝴蝶翼眼斑形成的分子机制

• 批准号: 2684638
• 负责人: David Lawrence Lewis
• 金额: $ 3.18万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2684638
• 关键词: Lepidoptera; developmental genetics; gene expression; pigmentation

Examination of the expression of various appendage-patterning genes in butterfly wings have shown that a few are expressed in patterns suggestive of roles in wing color pattern formation. For example, Distalless (Dll) gene expression has been found in cells destined to become the focus of the eyespot color pattern in both species of butterflies examined thus far, Precis coenia and Bicyclus anynana. One of the goals described in this proposal is to determine if the patterning gene Dll functions in eyespot formation. This will be accomplished by characterizing the effect of ectopic expression of the gene encoding Dll on eyespot formation and inhibiting the expression of Dll within the developing eyespot. Other genes involved in eyespot determination and formation will be identified using a PCR-based screen for genes expressed preferentially in the eyespot. The function of these genes will be tested using methodologies developed for testing the function of the Dll gene and in some cases, by implanting the gene product directly. These studies may reveal that the developmental pathways used to form butterfly wing patterns utilize the same mechanisms and genes used in determining body and appendage patterns in insects and vertebrates. Thus, given the tremendous variety of butterfly wing patterns, studying their development would provide a deeper understanding of the mechanisms guiding intricate cellular pattern development in other animals.

各种附属物模式基因表达的检查 蝴蝶翅膀已经表明，有一些是用图案来表达的 暗示在翅膀颜色图案形成中的作用。例如， 已在细胞中发现 Distalless (Dll) 基因表达 成为两个物种眼斑颜色图案的焦点 到目前为止，已经检查过的蝴蝶有 Precis coenia 和 Bicyclus anynana。之一 本提案中描述的目标是确定图案是否 基因 Dll 在眼斑形成中发挥作用。这将通过以下方式完成 表征编码 Dll 的基因异位表达的影响 影响眼斑形成并抑制 Dll 的表达 发育中的眼点。其他参与眼斑测定的基因 将使用基于 PCR 的表达基因筛选来鉴定形成 优先在眼点。这些基因的功能将被测试 使用为测试 Dll 基因功能而开发的方法 在某些情况下，通过直接植入基因产物。这些研究 可能揭示形成蝴蝶翅膀的发育途径 模式利用与确定身体相同的机制和基因 以及昆虫和脊椎动物的附属物模式。因此，鉴于 各种各样的蝴蝶翅膀图案，研究它们的发育 将提供对指导复杂的机制的更深入的理解 其他动物的细胞模式发展。