DROSOPHILA MISGUIDED GENE AND TRACHEAL CHEMOTAXIS

果蝇误导基因和气管趋化性

• 批准号: 2608727
• 负责人: Jill Jarecki
• 金额: $ 2.52万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2608727
• 关键词: Drosophilidae; arthropod genetics; cell differentiation; chemotaxis; developmental genetics; trachea

The long term objective of this research is to understand the molecular mechanisms regulating chemotaxis during terminal branch outgrowth in the tracheal system of Drosophila. The directed outgrowth of these cells is controlled by an attractive guidance signal originating from oxygen-starved target tissues. This is similar to the development of other epithelial tubes, such as the mammalian vascular system, in which the capillaries also develop in response to the oxygen needs of their target tissues. Therefore, the molecular characterization of the gene misguided, which has misrouted terminal branches, as well as the identification of other genes affecting the directed outgrowth of these cells will help elucidate the molecular mechanisms controlling terminal cell outgrowth. In the long term, this type of molecular genetic approach will hopefully identify the tracheogenic signals originating from the target issues, lead to a greater understanding of the role of oxygen in regulating these signals, determine the manner in which these signals are communicated to and transduced by the terminal cells, and elucidate the cellular mechanisms used during cytoplasmic outgrowth of the terminal branches. Studying how the physiological requirements of the target tissues are coupled to the development of the terminal cells in Drosophila will also lead to a molecular understanding of this process in other systems that are less amenable to molecular genetics.

这项研究的长期目标是了解 在终末分支生长过程中调节趋化性的机制 果蝇的气管系统。 这些细胞的定向生长是 由来自缺氧的有吸引力的引导信号控制， 靶组织。 这与其他上皮细胞的发育相似。 管，如哺乳动物的血管系统，其中毛细血管还 是为了满足其目标组织的氧气需求而产生的。 因此，该基因的分子特征被误导， 错误的终端分支，以及其他基因的识别 影响这些细胞的定向生长将有助于阐明 控制终末细胞生长的分子机制。 从长远 长期而言，这种类型的分子遗传学方法将有望确定 气管信号起源于目标问题，导致更大的 了解氧气在调节这些信号中的作用，确定 这些信号传递给和转换的方式 终末细胞，并阐明细胞机制， 顶生枝的细胞质向外生长。 研究如何 靶组织的生理需求与靶组织的生理需求相关联。 果蝇的终末细胞的发育也会导致 在其他系统中对这一过程的分子理解 适合分子遗传学。