RETINAL ORGANIZATION OF COLOR PATHWAYS

描述（改编自申请人摘要）：问题是 神经节细胞向大脑发送颜色信号的问题尚未解决。 M和P 构建体提供了由parasol细胞编码的无色信息， 和彩色信息的侏儒细胞，投影，分别， LGN的M和P层。 然而，在LGN的K层，每个M的腹侧 和P层，投射到富含细胞色素氧化酶的蓝/黄， 在VI的层2和3中的红/绿色颜色斑点，并且已知在这种情况下 蓝/黄通道，有蓝/黄小bistatively 神经节细胞（即，不是侏儒细胞）投射到LGN K细胞。 本提案的中心假设是， 神经支配LGN中的其他K细胞（即，α-CamK阳性γ和 （1）光电池足以提供额外的视觉通道， (2)包括神经节细胞，其汇集来自M 和L视锥细胞通过对立的双极细胞来支持红/绿色色觉。 在猕猴视网膜中检验这一假设的具体目的是：（1） 测量伽玛和伽玛细胞的空间分布， 视网膜的α-CamK染色;（2）建立其突触前 电路通过电子显微镜的连续切片，通过染色和 注射的细胞;和（3）确定谷氨酸受体的定位 双极细胞树突通过电子显微镜的连续切片， 染色并注射细胞。

DESCRIPTION (Adapted from applicant's abstract): The question of which ganglion cells send color signals to the brain is unresolved. The M and P construct provides that achromatic information is encoded by parasol cells, and chromatic information by midget cells that project, respectively, to the M and P layers of the LGN. However, K layers in the LGN, ventral to each M and P layer, project to the cytochrome oxidase-rich blue/yellow and red/green color blobs in layers 2 and 3 of VI, and it is known in the case of the blue/yellow pathway, that there are blue/yellow small bistratified ganglion cells (i.e., not midget cells) that project to the LGN K cells. The central hypothesis of the present proposal is that ganglion cells that innervate other K cells in the LGN (i.e., alpha-CamK-positive gamma and epsilon cells) (1) are sufficient to provide additional vision channels, and (2) include ganglion cells that pools spatially co-extensive signals from M and L cones via an opponent bipolar cell to underlie red/green color vision. The specific aims to test this hypothesis in macaque retina are: (1) to measure the spatial distribution of gamma and epsilon cells using alpha-CamK-staining of the retina; (2) to establish their presynaptic circuitry via electron microscopy of serial sections through stained and injected cells; and (3) to determine the localization of glutamate receptors to bipolar cell dendrites via electron microscopy of serial sections through stained and injected cells.