GLUCOCORTICOID CONTROL OF LEYDIG CELL DEATH AND MITOSIS

描述 本项目的长期目标是确定机制 GC抑制男性生殖功能。 GC以前曾 显示直接在睾丸水平上起作用，并抑制 编码睾酮生物合成酶的基因的表达。 GCS 也显示出控制细胞增殖的过程， 其他细胞类型的凋亡。 第一和第二个具体目标是 我们的建议是检查GC在体内或体内给药的效果， 对睾丸间质细胞有丝分裂和凋亡的影响。 第三个具体目标 建议是确定c-jun在Leydig细胞中的表达水平， 在体内和体外用GC处理。 在所有具体目标中，细胞将 由跨越关键发育阶段的3个不同年龄的大鼠制备 睾丸间质细胞成熟，并将与GC的水平， 反映了正常的环境或预期下， 压力的条件。

DESCRIPTION The long-term objectives of this project are to determine the mechanism(s) whereby GCs suppress male reproductive function. GCs have been previously show to have actions directly at the level of the testis, and to inhibit the expression of genes which encode testosterone biosynthetic enzymes. GCs have also been shown to control processes of cell proliferation and apoptosis in other cell types. The first and second specific aims of the proposal are to examine the effects of GCs administered either in vivo or in vitro on Leydig cell mitosis and apoptosis. The third specific aim of the proposal is to determine the level of c-jun expression in Leydig cells after treatment with GCs in vivo and in vitro. In all specific aims, cells will be prepared from rats of 3 different ages which span key development stages of the Leydig cell maturation, and will be treated with levels of GC which reflect either the normal milieu or that which would be expected under conditions of stress.