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STRUCTURAL INVESTIGATION OF NOVEL MUTANT HEMOGLOBINS

新型突变血红蛋白的结构研究

基本信息

批准号：
2765242
负责人：
金额：
$ 0.08万
依托单位：
MELLON PITTS CORPORATION (MPC CORP)
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/2765242
关键词：
bioengineering /biomedical engineering biological products biomaterials biomedical resource proteins

项目摘要

Our laboratory has developed an E. Coli expression system for production of recombinant novel mutant hemoglobins induced by site-specific mutagenesis. One objective of this system is to design mutants that will allow us to assign resonance peaks in NMR spectra of human hemoglobin. We have thus designed and produced surface a-His to Gin mutant hemoglobins that have enabled us to assign some aromatic resonances. Some mutant hemoglobins have shown decrease in oxygen cooperativity, suggesting significant structural perturbation at the intersubunit region of hemoglobin tetramer. This is interesting, since the amino acid substitution is on the surface of the hemoglobin, ~30 away from the intersubunit region, too far for anyone to suspect such prominent influence at the interface of the hemoglobin tetramer. We would like to investigate how a single amino acid substitution at the surface perturbs the interior sites of hemoglobin. We propose to use molecular dynamics to simulat e the structural changes. We plan to use the stochastic boundary molecular dynamics method(SBMD) for simulation to reduce the processing time. We may have to resolve to including the entire protein in the calculation, however, since it is difficult to design a boundary that will include both the mutation site at the surface and the atoms at the intersubunit surface.

本实验室研制了一种E.大肠杆菌表达系统诱导的重组新突变血红蛋白的产生定点诱变。该系统的一个目标是设计突变体，这将使我们能够分配共振峰的NMR光谱，人血红蛋白。因此，我们设计并生产了表面a-His，杜松子酒突变血红蛋白，使我们能够分配一些芳香族共振某些突变血红蛋白显示氧含量减少协同性，这表明显着的结构扰动，血红蛋白四聚体的亚基间区域。这很有趣由于氨基酸取代是在血红蛋白的表面上， ~30 远离亚基间区域，远到没人怀疑在血红蛋白四聚体的界面处如此显著的影响。我们想研究一个单一的氨基酸替换是如何在表面扰动血红蛋白的内部位点。我们建议用分子动力学来模拟结构变化。我们计划用随机边界分子动力学方法（SBMD）模拟，以减少处理时间。我们可能要下定决心在计算中包括整个蛋白质，因为它是很难设计一个既包含突变表面的原子和亚基间表面的原子。