INFERIOR COLLICULUS CALCIUM CURRENT AND SEIZURE

癫痫是一种神经元兴奋性障碍。在手机上 水平，发作活动由去极化电压串组成 需要钙(Ca)内流的移位(DS)。长期的 该项目的目标是识别细胞和分子 癫痫发作活动背后的蛋白质变化。这样做的目的是 建议是对钙电流的哪些方面进行表征 酒精戒断(ETX)和听源性惊厥(AGS)后的变化 继ETX之后，一种全身性癫痫模型。的具体目标 本研究的建议是：1)从药理和生物物理学角度 急性分离下壁细胞钙电流类型的识别 用膜片钳技术研究丘(IC)神经元，2)表征 电压对IC中N型和L型钙通道活动的影响 神经元3)观察ETX事件和AGS对钙电流的影响 4)确定在IC中表达哪些钙通道亚基 4)确定ETX和/或AGS是否改变钙亚基的分子技术 M RNA水平。确定钙内流在ETX发病机制中的作用 IC中的癫痫发作可能与临床上的全身性癫痫发作有关 强直-阵挛运动行为。了解细胞和分子 癫痫的基础将改进治疗策略和收益 对大脑功能和结构的新见解。

Epilepsies are disorders of neuronal excitability. At the cellular level, seizure activity consists of trains of depolarizing voltage shifts (DS) which requires calcium (Ca) influx. The long-term objectives of this project are to identify the cellular and molecular changes in proteins underlying seizure activity. The aim of this proposal is to characterize which aspects of Ca currents have been changed after ethanol withdrawal (ETX), and audiogenic seizure (AGS) following ETX, a model of generalized epilepsy. The specific aims of this research proposal are 1)to pharmacologically and biophysically identify the types of Ca currents in acutely dissociated inferior colliculus (IC) neurons using patch-clamp techniques, 2) to characterize the effects of voltage on N- and L-type Ca channel activity in IC neurons 3) to examine the effects of ETX episode, and AGS on Ca currents 4) to identify which Ca channel subunit are expressed in the IC using molecular techniques 4) to determine if ETX and/or AGS alter Ca subunit mRNA levels. Determining a role for Ca influx in the mechanism of ETX seizure in the IC may be relevant to clinical generalized seizure with tonic-clonic motor behaviors. Understanding the cellular and molecular basis of epilepsy will improve therapeutic strategies as well as yield new insights into brain function and structure.