EIF4E OVEREXPRESSION AND BREAST CANCER RECURRENCE

EIF4E 过度表达与乳腺癌复发

• 批准号: 2869821
• 负责人: BENJAMIN D LI
• 金额: $ 10万
• 依托单位: LOUISIANA STATE UNIV HSC SHREVEPORT
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-18 至 2000-09-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2869821
• 关键词: biomarker; breast neoplasms; cancer risk; clinical research; estrogen receptors; female; gene expression; genetic susceptibility; human subject; neoplasm /cancer relapse /recurrence; progesterone receptors; prognosis; translation factor; western blottings; women's health

The investigators propose to determine eukaryotic initiation factor 4E, which assists RNA helicases to bind to 51 untranslated regions of mRNA. Overexpression in cell lines produces a malignant phenotype. In preliminary studies, overexpression determined by Western blot occurs in breast cancers, but not in benign breast lesions. Preliminary data also associates the degree of overexpression with tumor aggressiveness and survival. However, these preliminary studies are based on small numbers. Thus, the investigators propose to perform a prospective study of 168 women with stage II or III breast cancer. Their first aim is to assess eIF4E overexpression in breast tissue, and their second aim is to perform ER and PR analysis so as to be able to relate this data to the eIF4E. They believe that in 5 years, they will have enough statistical power to determine if overexpression is associated with recurrence. The investigators believe that this data might lead to more informed decisions regarding who needs chemotherapy, i.e., by implication that if eIF4E is predictive of recurrence in stage II, then it might be for stage I as well. Then, a much larger study with sufficient power for determining recurrence in stage I breast cancer will be justified.

研究人员建议确定真核起始因子4E， 其帮助RNA解旋酶结合mRNA的51个非翻译区。 细胞系中的过表达产生恶性表型。 在 初步研究，通过Western印迹确定的过表达发生在 在乳腺癌中，而不是在良性乳腺病变中。 初步数据 也将过度表达的程度与肿瘤的侵袭性联系起来 和生存然而，这些初步研究是基于小 号码因此，研究人员建议进行一项前瞻性研究， 168名患有II期或III期乳腺癌的女性。 他们的首要目标是 评估eIF4E在乳腺组织中的过度表达，他们的第二个目的是 进行ER和PR分析，以便能够将这些数据与 eIF4E。 他们相信5年后， 统计功效，以确定过表达是否与 复发 研究人员认为，这些数据可能会导致更多的 关于谁需要化疗的知情决定，即，通过 这意味着如果eIF4E预测II期复发，那么 也可能是第一阶段。然后，一项更大的研究， 确定I期乳腺癌复发的充分把握度 将是合理的。