TUNABLE IR LASER MASS SPECTROMETER FOR DNA ANALYSIS

基质辅助激光解吸电离质谱仪(MALDI-MS) 在多核苷酸分析方面具有独特的潜力，超越了 其他软电离方法，因为它能够引入大量的 寡核苷酸分子进入气相，样品降解最少。 我们已经提出了一种可调谐的红外激光器来扩展这种能力，通过 探索各种解吸矩阵和协议以克服 低电离效率和亚稳态衰变 常规UV-MALDI-MS在第一阶段，我们已经构建了一个新颖的、可调的IR 由光学参量振荡器(OPO)组成的光源 可调谐的Cr：LiSAF激光器，获得的高分辨率增量m小于 在Mu/z=5735时，S/N大于DNAIR-MALDI质谱图， 用这种激光处理蛋白质和多肽。继此之后，成功地 可行性研究，我们将在第二阶段建造一个原型紧凑型IR- 具有分析大生物分子独特潜力的MALDIMS仪器 在高吞吐量下。这台仪器将积分一个延迟的离子 用二极管泵浦的可调谐的Cr：LiSAF/0P0激光器提取T0F-MS 2.7MU至3.4MU，可在较大范围内激发O-H、C-H和N-H键 矩阵。利用该仪器进行MALDI的DNA分析及优化 技术将会被执行。这项工作将有助于开发新功能 MALDI-MS对生物医学分析的基本影响。 建议的商业应用： 许多医疗和临床应用，如dna和rna分析，聚合酶链式反应。 片段，蛋白质和多肽，是设想的。DNA诊断学 应用程序将使紧凑、经济高效的 MALDI-MS系统，用于医疗保健、医药、农业和 环境监测领域。

Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI-MS) has a unique potential for polynucleotide analysis beyond the limits of other soft ionization methods, because of its ability to introduce large oligonucleotide molecules into gas phase, with minimum sample degradation. We have proposed a tunable infrared laser to expand this capability, by exploring a variety of desorption matrices and protocols for overcoming low ionization efficiency and metastable decay encountered with conventional UV-MALDI-MS. In Phase I, we have built a novel, tunable, IR source consisting of an optical parametric oscillator (OPO) pumped by a tunable Cr:LiSAF laser, and obtained high resolution delta m is less than 15 at mu/z=5735, S/N is greater than l50) IR-MALDI mass spectra of DNA, protein and peptides with this laser. Following this successful feasibility study, we will build, in Phase II, a prototype compact IR- MALDIMS instrument having unique potential to analyze large biomolecules at high throughput. This instrument will integrate a Delayed-ion Extraction T0F-MS with diode-pumped Cr:LiSAF/0P0 laser widely tunable from 2.7 to 3.4 mu, which can excite O-H, C-H and N-H bonds in wide range of matrices. Using this instrument DNA analysis and optimization of MALDI techniques will be performed. This work will help develop new features of MALDI-MS with fundamental impact on biomedical analysis. PROPOSED COMMERCIAL APPLICATION: Many medical and clinical applications such as, analysis of DNA & RNA, PCR fragments, proteins and peptides, are envisaged. The DNA diagnostics application will enable commercialization of compact, cost effective, MALDI-MS systems for use in health-care, medicine, agriculture, and environmental monitoring fields.