GENERATION OF A RAT EST (REST) CATALOG

人类基因组计划的一个主要目标是鉴定 一整套人类基因单次通过，cDNA部分测序 克隆产生EST提供了一种快速和成本有效的方法， 基因发现 与此同时，序列信息包含 为基因定位提供了关键资源。 了解新发现的人类基因的功能需要 越来越多地使用模型生物， 被操纵了 从模式生物中产生EST， 这些EST的比较基因组图谱也可以用于 人类疾病基因狩猎，以及调查大规模的 基因组的结构和组织。 老鼠是一个重要的 研究许多人类遗传基础的实验模型 疾病和一系列广泛的遗传育种的老鼠品系与疾病 特性可用。 因此，如果能够产生 大鼠基因组的EST目录和唯一基因索引。这 申请提出了以下具体目标：（1）生成大鼠 EST目录（REST）通过随机取样cDNA克隆， 不同组织的标准化大鼠cDNA文库。 (2)构建非 标准化的全长大鼠cDNA文库，并采用微阵列 技术.

A major goal of the human genome project is identification of the complete set of human genes. Single-pass, partial sequencing of cDNA clones to generate ESTs provides a rapid and cost-effective method of gene discovery. At the same time, sequence information contained within ESTs provides a key resource for gene mapping. Understanding the function of newly identified human genes requires the increased use of model organisms that can be genetically manipulated. The generation of ESTs from model organisms and comparative genome mapping of these ESTs can also be exploited for human disease gene hunting, as well as investigating the large-scale structure and organization of the genome. The rat is an important experimental model for studying the genetic basis of many human diseases and a wide array of genetically bred rat strains with disease characteristics are available. Hence, it would be beneficial to generate an EST catalog and unique gene index of The rat genome. This application proposes the following specific aims: (1) Generate a rat EST catalog (REST) by randomly sampling cDNA clones from normalized rat cDNA libraries of different tissues. (2) Construct non- normalized full-length rat cDNA libraries and employ micro array technology.