MRNA SPLICING FACTOR BRR2

mRNA 剪接因子 BRR2

• 批准号: 2872615
• 负责人: STEPHEN D RADER
• 金额: $ 3.57万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2872615
• 关键词: RNA binding protein; RNA splicing; enzyme structure; enzyme substrate; genetic regulation; helicase; intermolecular interaction; messenger RNA

Pre-mRNA splicing. The process of removing non-coding sequences (introns) from the transcripts of genes, is essential for viability in higher Eukaryotes. The splicing process involves hundreds of proteins and multiple RNA species which must come together in a precise sequence at the correct times, in order to remove the introns and nothing else. The multiple rearrangements and conformational changes that occur during splicing are hypothesized to be mediated by, among other things, RNA helicases, a class of proteins that unwind specific regions of RNA. The goal of this proposal is to investigate the role of a specific splicing factor, Brr2, in the splicing process and in the rearrangements that occur during splicing. One mutation in Brr2 blocks the first step in pre- mRNA splicing, and sequence similarity suggests that it is an RNA helicase. Furthermore, there is evidence that it interacts directly with the U2 snRNP. Implying that the latter may be its substrate. The experiments proposed here will identify the substrate of Brr2. look for its regulators, and define the function of a putative dsRNA-binding domain in Brr2. This work will extend our biochemical understanding of this crucial class of proteins and provide a foundation for future structural studies of helicases.

Pre-mRNA拼接。去除非编码序列（内含子）的过程

项目成果

Targeting of U4/U6 small nuclear RNP assembly factor SART3/p110 to Cajal bodies.

将 U4/U6 小核 RNP 组装因子 SART3/p110 靶向卡哈尔体。

• DOI: 10.1083/jcb.200210087
• 发表时间: 2003-02-17
• 期刊: The Journal of cell biology
• 作者: Stanĕk D;Rader SD;Klingauf M;Neugebauer KM
• 通讯作者: Neugebauer KM