FLOW CYTOMETRIC ANALYSIS OF GENOTOXICITY

遗传毒性的流式细胞术分析

• 批准号: 6340516
• 负责人: JOHNATHAN C SHARPE
• 金额: $ 11.45万
• 依托单位: CYTOMATION GTX, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-18 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6340516
• 关键词: DNA damage; cell line; cytotoxicity; flow cytometry; method development; surface antigens

DESCRIPTION: (Adapted from applicant's abstract): The objective of this proposal is to develop a low cost, rapid, reliable and sensitive flow method to determine mutagenesis. The system is based on a CHO AL cell line, which has human chromosome 11. Mutations in chromosome 11 are detected by loss of expression of surface antigens encoded on chromosome 11. The hypothesis is that mutations in chromosome 11 can be accurately, sensitively and rapidly detected by flow cytometry based on the loss of surface antigens. The specific aims of this proposal are to develop optimal methods for staining CHO AL cells with antibodies to measure the presence or absence of S1 antigen: determine the sensitivity of the flow cytometry mutagenesis assay; validate the assay by comparing mutant fractions measured with flow cytometry to that found by the standard assay; and identify design parameters for a small, reliable inexpensive, high resolution, low noise, fast flow cytometer to make routine measurements of mutant frequency. The development of this methodology would allow for rapid and inexpensive screening pharmaceuticals for genotoxicity. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述：（改编自申请人的摘要）：目的是