QUANTITATIVE FISH IMAGE ANALYSIS

定量鱼类图像分析

• 批准号: 2872034
• 负责人: MARK A SCHULZE
• 金额: $ 7.94万
• 依托单位: ADVANCED DIGITAL IMAGING RESEARCH, LLC
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-15 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2872034
• 关键词: bioimaging /biomedical imaging; biomedical automation; biomedical equipment development; cytogenetics; digital imaging; fluorescent in situ hybridization; human genetic material tag; image processing; nucleic acid quantitation /detection; nucleic acid sequence; telomere

DESCRIPTION: (Adapted from the investigator's abstract) This project aims to develop automated instrumentation and digital image analysis techniques to support the quantification of specific FISH-labeled DNA sequences in interphase and metaphase nuclei. The proposed approach combines digital image correction methods with off-slide and on-slide standards to obtain accurate total fluorescence measurements for FISH-labeled structures. Surface fitting and background subtraction will be used for image flattening, grayscale linearization and normalization, and color compensation. In Phase I the PI plans to determine the accuracy with which FISH can be quantified, and in Phase II the PI plans to develop and evaluate a prototype instrument. Phase III will involve the commercialization of the technology in PSI's existing product line of laboratory automation instruments. The PI foresees an application for this technology in research on the role of telomeric DNA in cancer and aging, in gene expression studies in cancer research and diagnosis, and in the detection of subtle deletions in clinical cytogenetics. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述：(改编自调查人员的摘要)本项目旨在 开发自动化仪器和数字图像分析技术以 支持间期特定FISH标记DNA序列的量化 和中期核。所提出的方法结合了数字图像校正 方法用载玻片外标准和载玻片上标准获得准确的总计数 鱼标记结构的荧光测量。曲面拟合和 背景减去将用于图像平坦化、灰度 线性化和归一化，以及颜色补偿。在第一阶段中，PI 计划确定鱼类可以量化的准确度，并分阶段 PI计划开发和评估一个原型仪器。第三阶段将 参与PSI现有产品线的技术商业化 实验室自动化仪器。PI预计会有这方面的应用 端粒DNA在癌症和衰老中作用的研究技术 基因表达研究在癌症研究和诊断以及检测中的应用 临床细胞遗传学中的微妙缺失。 建议的商业应用：不可用