NOVEL HOST GENES AFFECTING TRANSPOSITION

影响转座的新宿主基因

• 批准号: 2713751
• 负责人: JILL B KEENEY
• 金额: $ 2.62万
• 依托单位: JUNIATA COLLEGE
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-06-01 至 2000-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2713751
• 关键词: Saccharomyces cerevisiae; fungal genetics; gene deletion mutation; gene expression; gene interaction; genetic transduction; host organism interaction; molecular cloning; protein structure function; temperature; transposon /insertion element; virus infection mechanism; viruslike particle; western blottings

An understanding of retroviral mechanisms is of interest to the medical community and the general public, because of the role of viruses in infections (such a HIV and other T-cell viruses) and cancer that result from transfer and misregulation of proto-oncogenes. The proposed project is to identify yeast genes involved in the transposition cycle of the yeast retroelement Ty1. The Ty1 element transposes via a mechanism bearing many similarities to retroviruses which infect larger eukaryotes. Ty1 is a good model system, as dissection of the Ty1 transposition pathway is facilitated by the ease of yeast molecular genetic techniques. Transposition of Ty1 is known to be temperature sensitive. The immediate goals of this project are to determine the effects of temperature on the transposition process, and to clone mutant yeast genes which allow transposition to occur at elevated temperatures. The long-term goal for the project will be to determine the function of these cloned host genes in the transposition process. Comparative biochemical studies of Ty1 virus-like particles (VLPs) isolated from mutant and wild-type strains will enable the determination of their role in transposition. If the cloned genes have not been previously studied, genetic studies will be initiated to determine the cellular function of the genes. Long-term objectives beyond this proposal period will be to identify the counter- parts of the cloned genes in larger eukaryotes. This will give clues as to how viruses utilize host genes and may eventually allow for strategic interference of viral life-cycles.

了解逆转录病毒的机制是有兴趣的医学 社区和公众，由于病毒在 感染（如HIV和其他T细胞病毒）和癌症， 原癌基因的转移和失调拟建项目 是确定参与转座周期的酵母基因， 酵母逆转录元件Ty1。Ty1元件通过机械轴承进行换位 与感染较大真核生物的逆转录病毒有许多相似之处。Ty1是 一个很好的模型系统，因为Ty1转座途径的解剖是 酵母分子遗传技术的便利。 已知Ty1的转座是温度敏感的。立即 该项目的目标是确定温度对 转座过程，并克隆突变酵母基因， 在升高的温度下发生转座。年远景目标 该项目将确定这些克隆的宿主基因的功能 in the transposition转换process处理. Ty1的比较生物化学研究 从突变株和野生型菌株中分离的病毒样颗粒（VLP） 将能够确定它们在换位中的作用。如果 克隆基因以前没有研究过，遗传学研究将是 开始确定基因的细胞功能。长期 本提案期后的目标将是确定反 在较大的真核生物中克隆的部分基因。这将提供线索， 病毒如何利用宿主基因，并最终可能使战略 干扰病毒的生命周期。

项目成果

Increase in Ty1 cDNA recombination in yeast sir4 mutant strains at high temperature.

高温下酵母 Sir4 突变株中 Ty1 cDNA 重组增加。

• DOI: 10.1534/genetics.102.012708
• 发表时间: 2004
• 期刊: Genetics.
• 作者: Radford,SarahJ;Boyle,MeredithL;Sheely,CatherineJ;Graham,Joel;Haeusser,DanielP;Zimmerman,Leigh;Keeney,JillB
• 通讯作者: Keeney,JillB