NOVEL HOST GENES AFFECTING TRANSPOSITION
NOVEL HOST GENES AFFECTING TRANSPOSITION
批准号:
2713751
负责人:
JILL B KEENEY
金额:
$2.62万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1996
资助国家:
美国
项目状态:
已结题
起止时间:
1996-06-01 至 2000-05-31
中文摘要
了解逆转录病毒的机制对医学来说是很有意义的
英文摘要
An understanding of retroviral mechanisms is of interest to the medical
community and the general public, because of the role of viruses in
infections (such a HIV and other T-cell viruses) and cancer that result
from transfer and misregulation of proto-oncogenes. The proposed project
is to identify yeast genes involved in the transposition cycle of the
yeast retroelement Ty1. The Ty1 element transposes via a mechanism bearing
many similarities to retroviruses which infect larger eukaryotes. Ty1 is
a good model system, as dissection of the Ty1 transposition pathway is
facilitated by the ease of yeast molecular genetic techniques.
Transposition of Ty1 is known to be temperature sensitive. The immediate
goals of this project are to determine the effects of temperature on the
transposition process, and to clone mutant yeast genes which allow
transposition to occur at elevated temperatures. The long-term goal for
the project will be to determine the function of these cloned host genes
in the transposition process. Comparative biochemical studies of Ty1
virus-like particles (VLPs) isolated from mutant and wild-type strains
will enable the determination of their role in transposition. If the
cloned genes have not been previously studied, genetic studies will be
initiated to determine the cellular function of the genes. Long-term
objectives beyond this proposal period will be to identify the counter-
parts of the cloned genes in larger eukaryotes. This will give clues as to
how viruses utilize host genes and may eventually allow for strategic
interference of viral life-cycles.
期刊论文(1)
专著(0)
科研奖励(0)
会议论文
Increase in Ty1 cDNA recombination in yeast sir4 mutant strains at high temperature.
高温下酵母 Sir4 突变株中 Ty1 cDNA 重组增加。
DOI:
10.1534/genetics.102.012708
发表时间:
2004
期刊:
Genetics.
影响因子:
--
作者:
[Radford,SarahJ, Boyle,MeredithL, Sheely,CatherineJ, Graham,Joel, Haeusser,DanielP, Zimmerman,Leigh, Keeney,JillB]
通讯作者:
Keeney,JillB
The molecular function of RTT105, a regulator of Ty1 transposition in yeast
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批准号:7515838
-
项目类别:
-
资助金额:$18.28万
-
财政年份:2008
-
负责人:JILL B KEENEY
-
依托单位:
Mobility Control of a Yeast Retrotransposon
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批准号:6954302
-
项目类别:
-
资助金额:$19.44万
-
财政年份:2005
-
负责人:JILL B KEENEY
-
依托单位:
The role of SIR4 in Ty1 retrotransposition
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批准号:6504661
-
项目类别:
-
资助金额:$12.37万
-
财政年份:2002
-
负责人:JILL B KEENEY
-
依托单位:
NOVEL HOST GENES AFFECTING TRANSPOSITION
-
批准号:2430497
-
项目类别:
-
资助金额:$2.5万
-
财政年份:1996
-
负责人:JILL B KEENEY
-
依托单位:
NOVEL HOST GENES AFFECTING TRANSPOSITION
-
批准号:2193674
-
项目类别:
-
资助金额:$3.38万
-
财政年份:1996
-
负责人:JILL B KEENEY
-
依托单位:
海外基金