SENSITIVITY OF BIOASSAYS USING MAGNETIC MARKERS

我们建议设计，构造和演示一种基于 磁标记和超导量子干涉仪 检测器的目标是快速和灵敏的识别1-1000 生物测定中的生物颗粒。 基于磁性标签的生物测定 将减少或消除对放射性同位素的依赖， 从而损害颗粒检测的灵敏度。 磁性标记 也可以在不透明介质中检测到，例如血液或尿液，因为 信号与复杂介质的吸收特性无关。 使用SQUID，非特异性信号的显著减少 可以实现绑定。 用于快速检测和 识别少量的单个生物颗粒， 放射性和复杂样品中可能有广泛的应用， 生物医学研究、卫生保健、职业安全和水 监测. 拟定商业应用： 基于SQUID的个体生物快速识别仪 粒子可以在医学、职业医学、 安全、环保和国防， 生物战的潜在战场和恐怖主义威胁 剂.

We propose to design, construct, and demonstrate an instrument based on magnetic markers and superconducting quantum interference device (SQUID) detectors for the goal of rapid and sensitive identification of 1-1000 biological particles in bioassays. Bioassays based upon magnetic tags would reduce or eliminate the reliance on radio-isotopes without compromising on the sensitivity of particle detection. Magnetic markers can also be detected in opaque media, such as blood or urine, since the signal is independent of the absorptive properties of complex media. Using SQUIDs, a significant reduction in the signal from non-specific binding might be achieved. Instruments for rapidly detecting and identifying a small number of individual biological particles without radioactivity and in complex samples could have broad applications in biomedical research, health care, occupational safety, and water monitoring. PROPOSED COMMERCIAL APPLICATION: SQUID-based instrument for rapidly identifying individual biological particles could have major applications in medicine, occupational safety, environmental protection, and national defense by reducing the potential battlefield and terrorist threats of biological warfare agents.