SENSITIVITY OF BIOASSAYS USING MAGNETIC MARKERS

使用磁性标记的生物测定的灵敏度

• 批准号: 2713831
• 负责人: STUART J BERKOWITZ
• 金额: $ 9.97万
• 依托单位: CONDUCTUS, INC.
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2713831
• 关键词: bioassay; bioengineering /biomedical engineering; biomagnetism measurement; biomedical equipment development; superconductivity

We propose to design, construct, and demonstrate an instrument based on magnetic markers and superconducting quantum interference device (SQUID) detectors for the goal of rapid and sensitive identification of 1-1000 biological particles in bioassays. Bioassays based upon magnetic tags would reduce or eliminate the reliance on radio-isotopes without compromising on the sensitivity of particle detection. Magnetic markers can also be detected in opaque media, such as blood or urine, since the signal is independent of the absorptive properties of complex media. Using SQUIDs, a significant reduction in the signal from non-specific binding might be achieved. Instruments for rapidly detecting and identifying a small number of individual biological particles without radioactivity and in complex samples could have broad applications in biomedical research, health care, occupational safety, and water monitoring. PROPOSED COMMERCIAL APPLICATION: SQUID-based instrument for rapidly identifying individual biological particles could have major applications in medicine, occupational safety, environmental protection, and national defense by reducing the potential battlefield and terrorist threats of biological warfare agents.

我们建议设计、构建和演示一个基于 磁性标记和超导量子干涉装置(SQUID) 用于快速和灵敏地识别1-1000的探测器 生物检测中的生物颗粒。基于磁性标签的生物检测 将减少或消除对放射性同位素的依赖 降低了颗粒检测的灵敏度。磁性记号笔 也可以在不透明的介质中检测到，如血液或尿液，因为 信号与复杂介质的吸收特性无关。 使用SQUID，显著减少了来自非特定 可以实现绑定。用于快速检测和检测的仪器 识别少量单独的生物颗粒，而不是 放射性和复杂样品中的放射性可以在 生物医学研究、卫生保健、职业安全和水 监控。 建议的商业应用： 一种基于鱿鱼的生物个体快速识别仪 粒子可能在医学、职业等领域有重要应用 安全，环保和国防，通过减少 生物战的潜在战场和恐怖威胁 探员们。