QUANTITATIVE EPITOPE MAPPING OF IGA TO HIV GP120

IGA 与 HIV GP120 的定量表位作图

• 批准号: 2645271
• 负责人: STEPHEN L CARRITHERS
• 金额: $ 10.48万
• 依托单位: GENESIS BIOLOGICS, INC.
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-03 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2645271
• 关键词: HIV envelope protein gp120; enzyme linked immunosorbent assay; epitope mapping; human tissue; immunoglobulin A; laboratory mouse; method development

DESCRIPTION: Although significant progress has been made in the development of an HIV-1 vaccine, we are just now beginning to make progress toward the induction of mucosal immunity. A major limitation on the development of mucosal vaccines has been difficulty in quantitatively evaluating the immune response at mucosal surfaces. We propose to develop an assay which will provide a quantitative measure of epitope specific antibodies at the mucosa. It is our hypothesis that the induction of mucosal antibodies can be accurately measured using this method and that the information will lead to improved HIV-1 vaccines that will block the spread of infection. Our proposed Quantitative Epitope Mapping ELISA format, or QEM ELISA, provides a sensitive way of measuring the concentration of epitope specific antibody. The assay can be easily adapted to analysis of samples with widely varying levels of antibody including serum, saliva, feces, and urine with minor modifications of the assay format. The assay can also be used to measure isotype specific epitopes without the need to deplete the sample for competing Ig subclasses or other interfering substances. Long term the QEM ELISA will have broad application to the analysis of vaccines for other disease (e.g. rotavirus, RSV, influenza, H. pylori, HPV, etc.). PROPOSED COMMERCIAL APPLICATION: Not available.

描述：虽然在这方面已经取得了重大进展 HIV-1 疫苗的开发，我们现在才刚刚开始 诱导粘膜免疫的进展。 一个主要限制 粘膜疫苗的研发一直是个难题 定量评估粘膜表面的免疫反应。 我们 提议开发一种提供定量测量的测定方法 粘膜上的表位特异性抗体。我们的假设是 可以使用以下方法准确测量粘膜抗体的诱导 这种方法和信息将导致 HIV-1 的改善 阻止感染传播的疫苗。 我们提出的 定量表位作图 ELISA 格式或 QEM ELISA 提供了 测量表位特异性浓度的灵敏方法 抗体。该测定可以很容易地适应样品分析 抗体水平差异很大，包括血清、唾液、粪便和 尿液，对测定形式进行了微小的修改。 测定还可以 用于测量同种型特异性表位，无需耗尽 样品中是否存在竞争性 Ig 亚类或其他干扰物质。 从长远来看，QEM ELISA 将广泛应用于分析 其他疾病的疫苗（例如轮状病毒、RSV、流感、幽门螺杆菌、 HPV等）。 拟议的商业应用：不可用。