QUANTITATIVE EPITOPE MAPPING OF IGA TO HIV GP120

描述：尽管在以下方面取得了重大进展 开发HIV-1疫苗，我们现在才刚刚开始制造 粘膜免疫诱导的研究进展。一个主要的限制 关于黏膜疫苗的开发一直存在困难 定量评估粘膜表面的免疫反应。我们 建议开发一种可提供定量测量的化验方法 粘膜上的抗原表位特异性抗体。我们的假设是 粘膜抗体的诱生可以用 这一方法和信息将导致改进HIV-1 将阻止感染传播的疫苗。我们的建议 定量表位映射ELISA格式，或QEM ELISA，提供了一种 一种灵敏的表位特异性浓度测定方法 抗体。该分析方法可以很容易地适应于样品的分析 抗体水平差异很大，包括血清、唾液、粪便和 尿液，对化验格式稍作修改。该化验还可以 用于测量同种类型的特定表位，而不需要耗尽 竞争免疫球蛋白亚类或其他干扰物质的样本。 从长远来看，QEM-EL ISA将在血吸虫病的分析中有广泛的应用 其他疾病(如轮状病毒、呼吸道合胞病毒、流感、幽门螺杆菌、 HPV等)。 建议的商业应用：不可用。

DESCRIPTION: Although significant progress has been made in the development of an HIV-1 vaccine, we are just now beginning to make progress toward the induction of mucosal immunity. A major limitation on the development of mucosal vaccines has been difficulty in quantitatively evaluating the immune response at mucosal surfaces. We propose to develop an assay which will provide a quantitative measure of epitope specific antibodies at the mucosa. It is our hypothesis that the induction of mucosal antibodies can be accurately measured using this method and that the information will lead to improved HIV-1 vaccines that will block the spread of infection. Our proposed Quantitative Epitope Mapping ELISA format, or QEM ELISA, provides a sensitive way of measuring the concentration of epitope specific antibody. The assay can be easily adapted to analysis of samples with widely varying levels of antibody including serum, saliva, feces, and urine with minor modifications of the assay format. The assay can also be used to measure isotype specific epitopes without the need to deplete the sample for competing Ig subclasses or other interfering substances. Long term the QEM ELISA will have broad application to the analysis of vaccines for other disease (e.g. rotavirus, RSV, influenza, H. pylori, HPV, etc.). PROPOSED COMMERCIAL APPLICATION: Not available.