DEVELOPMENT OF A NOVEL HPV IDENTIFICATION SYSTEM

新型 HPV 识别系统的开发

• 批准号: 2645306
• 负责人: YING Z YANG
• 金额: $ 9.37万
• 依托单位: PROFILE DIAGNOSTIC SCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2645306
• 关键词: cancer risk; cervix neoplasms; communicable disease diagnosis; diagnosis design /evaluation; early diagnosis; genetic strain; human papillomavirus; nucleic acid hybridization; nucleic acid sequence; rapid diagnosis; recombinase; virus DNA; virus classification

DESCRIPTION (Adapted from investigator's abstract): Invasive carcinoma of the cervix is one of the most common cancers in the world. Human papillomavirus (HPV), progressing through cervical intraepithelial neoplasia, is the primary causal agent for the development of invasive cervical cancer. However, of the 70 or more HPV subtypes, few progress to cancer. Although early detection coupled with eradication of cervical cancer precursors has been shown to lead to a decreased incidence of invasive cervical cancer, the present state of the art employs diagnostic techniques that are too costly or too time consuming to screen large populations efficiently. Accurate HPV strain identification is presently only possible through cumbersome DNA hybridization analysis utilizing either Southern blot technology or in situ hybridization technology. In the following proposal, an enzyme- assisted, sequence-specific identification system will be developed. The simple, rapid, sensitive, accurate, and low-cost DNA diagnostic system described herein will detect HPV at very early stages of infection and will distinguish between low oncogenic risk strains and high oncogenic risk strains. This will enable large scale screening by establishing a diagnostic protocol that is sensitive, rapid, and relatively inexpensive. The diagnostic tool described by this proposal is also applicable to detection of other infectious diseases and genetic diseases with only minor modifications. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述（改编自研究者摘要）：浸润性癌