CONSTRUCTION OF A HUMAN OOCYTE CDNA LIBRARY

人卵母细胞CDNA文库的构建

• 批准号: 2540802
• 负责人: KAREN K YAMAMOTO
• 金额: $ 10万
• 依托单位: REPROGEN, INC.
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-03-15 至 1998-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2540802
• 关键词: biomarker; cell differentiation; complementary DNA; developmental genetics; egg /ovum; gene expression; genetic library; human genetic material tag; human tissue; messenger RNA; oogenesis; polymerase chain reaction

Studies on the role of specific molecules in the human fertilization process and additional assessment of potential applications for these proteins are hampered by the limited amounts of available biological material. The ability to isolate and characterize mRNA populations from human oocytes at different stages of oogenesis would facilitate the identification and characterization of genes involved in oogenesis, oocyte maturation, fertilization and embryo implantation. The technical objectives of the Phase I proposal are directed towards the construction and characterization of a human oocyte cDNA library and Serial Analysis of Gene Expression (SAGE) catalog. Once feasibility has been determined, additional developmental stage-specific oocytes SAGE catalogs will be constructed and used for the identification of novel stage-specific biomarkers, factors critical for oocyte maturation, and candidate targets for diagnostic tests for infertility and the design for contraceptives. PROPOSED COMMERCIAL APPLICATIONS: Commercial applications include the production of an oocyte cDNA library and SAGE catalog as well as the identification of novel candidate targets that might be used for contraceptive development, infertility diagnosis, genetic disease of embryonic development analysis, human oogenesis staging and in vitro maturation and fertilization.

特异性分子在人类受精过程中作用的研究 对这些潜在应用的进一步评估 蛋白质受到有限数量的可用生物 材料分离和表征mRNA群体的能力， 卵子发生不同阶段的人类卵母细胞， 鉴定和表征涉及卵子发生、卵母细胞 成熟、受精和胚胎植入。技术 第一阶段建议的目标是建设 人卵母细胞cDNA文库的构建、鉴定及系列分析 基因表达（SAGE）目录。一旦确定了可行性， 其他发育阶段特异性卵母细胞SAGE目录将 构建并用于鉴定新的阶段特异性 生物标志物、卵母细胞成熟的关键因素和候选靶点 用于不孕症的诊断测试和避孕药的设计。 拟议的商业应用： 商业应用包括卵母细胞cDNA文库的生产 和SAGE目录以及新的候选靶点的鉴定 可以用于避孕药的开发，不孕症的诊断， 胚胎发育遗传病分析，人类卵子发生分期 以及体外成熟和受精。