NON-INVASIVE ASSAY OF PREIMPLANTATION EMBRYO VIABILITY

植入前胚胎活力的非侵入性测定

• 批准号: 2772066
• 负责人: DAVID KEEFE
• 金额: $ 10.25万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2772066
• 关键词: age at pregnancy; biomedical equipment development; calcium flux; computer assisted diagnosis; computer program /software; cytodiagnosis; cytotoxicity; diagnosis design /evaluation; early embryonic stage; embryo /fetus culture; embryo /fetus monitoring; embryo /fetus transplantation; embryo implantation; female; fluorescence microscopy; fluorescent dye /probe; growth /development; hamsters; histology; noninvasive diagnosis

DESCRIPTION (Adapted from the applicant's abstract): This application seeks two years funding to support the development of instrumentation to assess the developmental potential of preimplantation embryos and to study the pathophysiology of oocyte dysfunction. The investigators are particularly interested in the study of age related oocyte dysfunction. The program involves a collaboration between the Director of the BioCurrents Research Center (MBL & NCRR), Dr. Peter J.S. Smith; and the Director of the IVF Clinic (Women and Infants) and Reproductive Medicine Laboratory (MBL), Dr. David Keefe. Central to this instrument development is the modification of established non-invasive, self-referencing, ion-selective probes to operate under environmental conditions capable of sustaining the long-term health of mammalian embryos. This will involve significant changes to present experimental platforms with longer term development of software algorithms to permit remote sensing of embryos and probe positions. The developing instrumentation will be simultaneously tested to explore the biological hypothesis that transmembrane calcium flux is an accurate predictor of embryo viability and oocyte dysfunction. The investigators anticipate this approach will be significantly more sensitive than predictors currently in clinical use. The Golden Hamster will be used as a model and data will be correlated with the age of the donor female, subsequent cell division in vitro and, ultimately, implantation success on transfer to a pseudopregnant recipient. The investigators expect the finished instrumentation to provide new insights into the basic physiology of mammalian oocytes and early embryos and to provide the foundation of clinically applicable assays for preimplantation embryo selection.

描述（改编自申请人的摘要）：本申请寻求 两年的资金，以支持仪器的发展，以评估 植入前胚胎的发育潜力，并研究 卵母细胞功能障碍的病理生理学。 调查人员特别 对年龄相关的卵母细胞功能障碍的研究感兴趣。 程序 涉及到生物电流研究中心主任 中心（MBL & NCRR），Peter J.S.博士史密斯;和IVF的主任 诊所（妇女和婴儿）和生殖医学实验室（MBL），博士。 大卫基夫。 该仪器开发的核心是修改 建立了非侵入性，自参考，离子选择性探针， 在能够维持长期健康的环境条件下， 哺乳动物胚胎 这将涉及目前的重大变化 长期开发软件算法的实验平台 以允许对胚胎和探针位置进行遥感。 发展中 仪器将同时进行测试，以探索生物 假设跨膜钙流是一个准确预测 胚胎活力和卵母细胞功能障碍。 调查人员预计， 这种方法将比目前的预测方法更敏感， 临床应用。 黄金海岸将被用作模型，数据将被 与供体女性的年龄相关，随后的细胞分裂， 体外，并最终植入成功转移到一个假孕 收件人。 研究人员希望完成的仪器能够提供 哺乳动物卵母细胞和早期胚胎的基本生理学的新见解 胚胎，并为临床适用的检测提供基础， 着床前胚胎选择