PEPTIDE HYDROLYSIS BY DINUCLEAR METAL COMPLEXES

本研究的目的是了解双核金属水解酶水解酰胺的机理，以及配位环境与金属中心活性之间的关系。这将通过使用合成模型复合物对双核金属水解酶的活性位点进行建模来完成。一个可调的，二核的酞嗪基配体系统已被设计用于本研究。这些配体的双核配合物将用于探索所提出的机制的各个方面，并且配体环境将被调整以优化水解活性。对具有水解活性的双核金属配合物的研究将有助于阐明金属水解酶的机制，阐明金属离子在这些体系中的作用，并最终有助于开发人工水解酶。

The objectives of this proposal are to gain an understanding of the mechanism of amide hydrolysis by dinuclear metallohydrolases and the relationship between the coordination environment and the activity of the metal center. This will be done by modeling the active sites of dinuclear metallohydrolase enzymes using synthetic model complexes. A tunable, dinucleating phthalazine-based ligand system has been designed for use in this study. Dinuclear complexes of these ligands will be used to probe aspects of the proposed mechanism, and the ligand environment will be tuned to optimize hydrolytic activity. This study of hydrolytically viable dinuclear metal complexes will aid in the elucidation of hydrolytic metalloenzyme mechanisms, clarify the roles of the metal ions in these systems, and ultimately aid in the development of artificial hydrolases.