X-RAY CRYSTALLOGRAPHY OF MACROMOLECULES

大分子的 X 射线晶体学

• 批准号: 2852388
• 负责人: ZBYSZEK OTWINOWSKI
• 金额: $ 33.86万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-05-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2852388
• 关键词: X ray crystallography; bioimaging /biomedical imaging; charge coupled device camera; computer program /software; computer system design /evaluation; image processing; method development; structural biology

DESCRIPTION (Adapted from abstract): The proposal is for continued development of the HKL suite of programs Denzo/Scalepack/XDisplayF for data reduction and scaling of x-ray diffraction data. New areas for development fall in three categories. Advancing the solution of the phase problem will be addressed by optimization of single-wavelength anomalous dispersion from sulfur, use of nonisomorphism as a source of phase information, and implementation of a method for calibration for CCD detectors, a generalized absorption correction, and a new interface for phasing tree analysis. Continued development of methods for improving data quality will be aimed at obtaining useful diffraction data from less than optimal crystals. The third area for development will be in standardizing optimal data collection strategies and data archiving.

描述（改编自摘要）：该提案用于继续开发 HKL的Denzo/Scalepack/XDisplayF程序套件用于数据缩减， X射线衍射数据的定标。新的发展领域分为三个 类别推进相位问题的解决将通过 硫的单波长反常色散的优化， 作为相位信息源的非同构和方法的实现 用于CCD探测器的校准，广义吸收校正，和 新的相位树分析界面。继续制定方法， 提高数据质量的目的是从以下方面获得有用的衍射数据： 低于最佳晶体。第三个发展领域将是 使最佳数据收集战略和数据存档标准化。