MOLECULAR DIAGNOSTIC TESTS FOR SIMIAN HERPESVIRUSES

猴子是生物医学研究的重要资源。 猿猴疱疹病毒 （B病毒; BV）对从事生物医学工作的人员来说是一种非常真实的危险。 和猕猴在一起 H. papio 2（HVP 2）在狒狒中也同样引起问题 繁殖殖民地 检测受感染猴子的灵敏检测方法是 需要识别对人员构成风险的动物， 用于建立无病毒繁殖菌落的无病毒动物。 可区分BV、HVP 2和人类的灵敏测定 单纯疱疹病毒（HSV）也是准确和快速地 人类感染猴病毒的鉴定。 为实现这些目标，将采取若干补充办法。 编码BV和HVP 2的病毒特异性gG糖蛋白的基因将被克隆。 克隆到诱导型表达载体中以建立稳定转化的 将用于开发敏感的病毒特异性ELISA的细胞， 检测BV和HVP 2抗体。 利用强抗体 对病毒衣壳蛋白（p40）的反应， 初次感染时，BV和HSV 1 p40基因将被克隆到 原核表达载体以产生重组蛋白， 开发免疫测定法，以提供早期血清学确认， 人类BV感染。 其他BV蛋白， 将鉴定和表达被猴血清识别的基因， 评价了开发诊断测试的潜在用途。 单克隆 还将生产、表征和使用BV抗体， 灵敏的免疫分析来识别感染的猴子和人类。 本项目将基础分子研究成果应用于临床， BV和HVP 2检测和诊断重要问题。 的测定 发展将允许快速，敏感和可靠的鉴别诊断 灵长类疱疹病毒感染，使灵长类种群管理者能够更多地 有效地运行设施，减少因 人畜共患疱疹感染。

Monkeys are a vital resource for biomedical research. Herpesvirus simiae (B virus; BV) presents a very real danger for biomedical personnel who work with macaques. H. papio 2 (HVP2) similarly causes problems in baboon breeding colonies. Sensitive assays for detection of infected monkeys are needed to identify animals which pose a risk to personnel and to identify virus-free animals for establishment of virus-free breeding colonies. Sensitive assays which can differentiate between BV, HVP2 and the human herpes simplex viruses (HSVs) are also necessary for accurate and rapid identification of human infections with monkey viruses. Several complementary approaches will be pursued to achieve these goals. Genes encoding the virus-specific gG glycoproteins of BV and HVP2 will be cloned into an inducible expression vector to establish stably transformed cells which will be used to develop sensitive, virus-specific ELISAs for detection of antibody to BV and HVP2. Capitalizing on a strong antibody response to the viral capsid protein (p40) which develops early following primary infections, the BV and HSV1 p40 genes will be cloned into a procaryotic expression vector to produce recombinant proteins for development of immunoassays to provide early serological confirmation of human BV infections. Additional BV proteins which are consistently recognized by monkey sera will be identified and expressed, and their potential use for development of diagnostic tests evaluated. Monoclonal antibodies to BV will also be produced, characterized, and used for sensitive immunoassays to identify infected monkeys and humans. This project applies results of basic molecular research to the clinically important problem of BV and HVP2 detection and diagnosis. The assays developed will permit rapid, sensitive and reliable differential diagnosis of primate herpesvirus infections, enabling primate colony managers to more effectively operate facilities and reduce loss of human life due to zoonotic herpes infections.