CORE--ANTIGEN AND ANTIBODY PRODUCTION

描述：（申请人描述）抗原和 抗体生产共享资源是提供及时的服务， 调查人员包括：1。GST融合蛋白的产生。2.鼠标 多克隆抗血清生产。3.小鼠单克隆抗体。4.单个 细胞克隆和亚克隆的冷冻保存。5.使用上述 用于鉴定特定基因产物的试剂。 对癌症易感性、进展和预后重要的基因是 正在以惊人的速度被确认。伴随这些发现的是 鉴定它们相互作用的蛋白质，这些蛋白质对 基因功能BRCA1和BRCA2的发现，以及随后， 证明它们与DNA修复蛋白Rad51和 Rad50在乳腺癌研究中强调了这些发现。的 及时开发纯化的融合蛋白有助于基因的研究 鉴定需要抗体的功能和产生 免疫染色定位蛋白质， 筛选癌症样本中的基因突变， proteins. 这个核心将提供一个简化的服务，一旦cDNA片段与 获得开放的阅读帧。由于GST融合蛋白通常用作 优良的抗原，我们将使用纯化的GST融合蛋白作为抗原 以产生多克隆和单克隆抗体。高质量多克隆 抗体，适用于免疫沉淀、免疫印迹和 免疫染色，将小鼠在无病原体的房屋中产生 设施。完善的杂交瘤核心设施将提供服务 单克隆抗体产品。核心小组还将就以下方面提供咨询意见： 蛋白质产品鉴定的方法和服务， 蛋白质产物的定位、免疫染色和图像分析， 用特异性抗体染色的细胞系或组织切片。以来 新鉴定的肿瘤抑制基因的研究或蛋白质产物， 信号分子和它们的相互作用分子 预后和诊断潜力，提供良好的表征 抗体及时将满足SACI的特殊需要 investigators.

Description: (Applicant's Description) The goal of the Antigen and Antibody Production Shared Resource is to provide timely services to investigators that include: 1. GST-fusion protein production. 2. Mouse polyclonal antisera production. 3. Mouse monoclonal antibody. 4. Single cell cloning and cryopreservation of the subclone. 5. The use of above reagents in characterizing specific gene products. Genes important for cancer predisposition, progression, and prognosis are being identified at a remarkable pace. Accompanying these findings is the identification of their interacting proteins that are important for the gene function. Discoveries of BRCA1 and BRCA2, and subsequently, demonstration of their interaction with the DNA repair proteins, Rad51 and Rad50, have highlighted such discoveries in breast cancer studies. The timely development of purified fusion proteins facilitates studies of gene function and production of antibodies are required for the identification of specific gene products, immunostaining to localize the protein, screening gene mutation in cancer samples, and studies of interacting proteins. This core will provide a streamlined service once a cDNA fragment with open reading frame is obtained. Since GST-fusion proteins usually serve as excellent antigens, we will use purified GST-fusion proteins as antigens to produce polyclonal and monoclonal antibodies. High quality polyclonal antibodies, suitable for immunoprecipitation, immunoblotting and immunostaining, will be produced in mice house in the pathogen-free facility. A well-established hybridoma core facility will provide services on monoclonal antibody products. The core will also provide advice on approaches and services for protein product identification and localization of protein product, immunostaining, and image analysis of cell lines or tissue sections stained with a specific antibody. Since studies or protein products of newly identified tumor suppressor genes, signaling molecules, and their interacting molecules have important prognostic and diagnostic potential, providing well-characterized antibodies in a timely manner will meet the special needs of the SACI investigators.