COLLAGEN GENE REGULATION IN DEVELOPMENT AND DISEASE
COLLAGEN GENE REGULATION IN DEVELOPMENT AND DISEASE
批准号:
2909796
负责人:
MICHAEL BREINDL
金额:
$22.42万
依托单位国家:
美国
项目类别:
财政年份:
1994
资助国家:
美国
项目状态:
已结题
起止时间:
1994-05-25 至 2003-04-30
关键词:
bone marrow cell differentiation chemical structure function chromatin collagen connective tissue development developmental genetics fibrosis gene expression genetic regulation genetic regulatory element genetically modified animals green fluorescent proteins introns laboratory mouse nucleic acid sequence nucleic acid structure protein structure function reporter genes tissue /cell culture transfection
中文摘要
描述:(改编自申请人的摘要)-I型胶原蛋白,
脊椎动物中含量最丰富的蛋白质,具有多种生物学功能,
包括为结缔组织提供拉伸强度,形成
所有主要内脏器官中结缔组织的框架,并促进
细胞在发育过程中的迁移和分化。 因此,
(but仍然缺乏)基因表达调控的知识
编码I型胶原蛋白对于理解这些不同的
以及可能影响它们的疾病。 申请人有长期
和成功的跟踪记录,分析5'侧翼序列的
a1(I)胶原基因。 正如申请人指出的,许多研究
试图解决细胞特异性调节表达的I型
胶原基因,但这些机制仍然不清楚。 特别是
a1(I)胶原基因和染色质远端元件的作用
结构未知。 因此,该提案的长期目标是
在分子水平上理解,参与的复杂机制,
阶段和a1(I)胶原基因的组织特异性调节。 的
申请人提出实现分析染色质的作用的目标
结构和核基质中a1(I)胶原表达的调控。
五个具体目标是:
因为细胞中高水平的a1(I)胶原蛋白产生大量的
I型胶原蛋白;识别和分析参与的调节元件,
a1(I)胶原结构域的染色质环组织;分析
转基因小鼠远端调控元件的功能;分析a1(I)
正常发育和分化中的调节元件,
纤维化疾病
英文摘要
DESCRIPTION: (Adapted from the applicant's abstract) - Type I collagen, the
most abundant protein in vertebrates, has diverse biologic function,
including providing tensile strength to connective tissues, forming the
framework of connective tissue in all major internal organs, and promoting
cell migration and differentiation during development. Thus, a detailed
(but still lacking) knowledge of the regulation of expression of the genes
encoding type I collagen is essential for understanding these various
processes and the diseases that can affect them. The applicant has a long
and successful track record in analyzing the 5' flanking sequence of the
a1(I) collagen gene. As the applicant points out, numerous studies have
tried to address the cell-specific regulation of expression of the type I
collagen genes yet these mechanisms are still not understood. In particular
the role of distal elements in the a1(I) collagen gene and of the chromatin
structure is unknown. Thus, the long-term goal of this proposal is to
understand at the molecular level, the complex mechanisms involved in the
stage and tissue-specific regulation of the a1(I) collagen gene. The
applicant proposes to achieve this goal of analyzing the role of chromatin
structure and the nuclear matrix in a1(I) collagen regulation of expression.
The five specific aims are: To identify regulatory elements that account
for the high level of a1(I) collagen in cells that produce large amounts of
type I collagen; to identify and analyze regulatory elements involved in the
chromatin loop organization of the a1(I) collagen domain; to analyze the
functions of distal regulatory elements in transgenic mice; to analyze a1(I)
regulatory elements in normal development and differentiation and in
fibrotic disorders.
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会议论文
COLLAGEN GENE REGULATION IN DEVELOPMENT AND DISEASE
-
批准号:2081085
-
项目类别:
-
资助金额:$15.19万
-
财政年份:1994
-
负责人:MICHAEL BREINDL
-
依托单位:
COLLAGEN GENE REGULATION IN DEVELOPMENT AND DISEASE
-
批准号:6511721
-
项目类别:
-
资助金额:$24.49万
-
财政年份:1994
-
负责人:MICHAEL BREINDL
-
依托单位:
COLLAGEN GENE REGULATION IN DEVELOPMENT AND DISEASE
-
批准号:2081086
-
项目类别:
-
资助金额:$14.9万
-
财政年份:1994
-
负责人:MICHAEL BREINDL
-
依托单位:
COLLAGEN GENE REGULATION IN DEVELOPMENT AND DISEASE
-
批准号:2081087
-
项目类别:
-
资助金额:$16.2万
-
财政年份:1994
-
负责人:MICHAEL BREINDL
-
依托单位:
COLLAGEN GENE REGULATION IN DEVELOPMENT AND DISEASE
-
批准号:6171290
-
项目类别:
-
资助金额:$23.09万
-
财政年份:1994
-
负责人:MICHAEL BREINDL
-
依托单位:
COLLAGEN GENE REGULATION IN DEVELOPMENT AND DISEASE
-
批准号:2413977
-
项目类别:
-
资助金额:$16.93万
-
财政年份:1994
-
负责人:MICHAEL BREINDL
-
依托单位:
COLLAGEN GENE REGULATION IN DEVELOPMENT AND DISEASE
-
批准号:2633650
-
项目类别:
-
资助金额:$24.01万
-
财政年份:1994
-
负责人:MICHAEL BREINDL
-
依托单位:
COLLAGEN GENE REGULATION IN DEVELOPMENT AND DISEASE
-
批准号:6374958
-
项目类别:
-
资助金额:$23.78万
-
财政年份:1994
-
负责人:MICHAEL BREINDL
-
依托单位:
CHARACTERIZE RETROVIRAL INTEGRATION TARGET SEQUENCES: CHROMATIN, GENE EXPRESSION
-
批准号:3934031
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:MICHAEL BREINDL
-
依托单位:
RETROVIRAL INTEGRATION SITE & INSERTIONAL MUTATION; MURINE TYPE I COLLAGEN GENE
-
批准号:3872053
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:MICHAEL BREINDL
-
依托单位:
RETROVIRAL INTEGRATION SITES & INSERTION MUTATION EFFECT ON TYPE I COLLAGEN GENE
-
批准号:3893712
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:MICHAEL BREINDL
-
依托单位:
COLLAGEN I GENE EXPRESSION & RETROVIRAL INSERTIONAL MUTAGENESIS: CHROMATIN
-
批准号:3912964
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:MICHAEL BREINDL
-
依托单位:
EUKARYOTIC GENE EXPRESSION AND INSERTIONAL MUTAGENESIS
-
批准号:3956650
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:MICHAEL BREINDL
-
依托单位:
海外基金