REACTIVITY OF NICKEL COMPLEXES WITH DNA

过渡金属络合物在化学研究中具有重要意义。 核酸的修饰。某些镍(II)络合物是 由于它们的致癌活性，人们特别感兴趣。这样做的目的是 建议理解与镍相关的化学事件 DNA损伤和研究镍肽是否可能被用作 高度特异的DNA识别和修饰剂。具体的 这项建议的目的是：1)确定序列的特异性和 过渡金属配合物与DNA相互作用的位置，2) 利用多肽配体模拟生理条件以探索 金属毒性，以及3)构建多肽文库以研究DNA 专一性。

Transition metal complexes are of considerable interest in the modification of nucleic acids. Certain nickel(II) complexes are of particular interest due to their carcinogenic activity. The goals of this proposal are to understand the chemical events related to nickel-mediated DNA damage and to investigate whether nickel peptides might be used as highly specific DNA recognition and modification agents. The specific aims of this proposal are to: 1) determine sequence specificity and the site of transition metal coordination compounds interaction with DNA, 2) mimic physiological conditions using peptide ligands in order to explore metal toxicity, and 3) generate peptide libraries to study DNA specificity.