UPREGULATION OF CKI P21 BY ANDROGEN IN PROSTATE CANCER

描述 前列腺癌是男性癌症死亡的第二大原因。 在早期阶段，前列腺癌的生长是雄激素依赖性的， 阉割是治疗前列腺癌的一种非常有效的方法。 前列腺癌雄激素非依赖性生长的发展 大约18个月的激素消融治疗是一个主要的临床 困境本研究的目的是探讨其分子机制 CKI p21/Cip 1/Waf 1在雄激素依赖性和非依赖性生长中的作用 前列腺癌细胞这项研究是基于我们的观察， p21/Cip 1/Waf 1基因在雄激素的作用下表达上调， 在雄激素非依赖性前列腺中发现了该基因的表达。 癌细胞系本提案将研究抗凋亡 p21/Cip 1/Waf 1基因在前列腺癌细胞中的功能， 以雄激素依赖性方式赋予细胞稳态， 前列腺癌细胞雄激素非依赖性生长的发展 增加基因的基础表达。的分子机制 将阐明雄激素对p21/Cip 1/Waf 1基因表达的作用。在 此外，还将建立体内雄激素非依赖性前列腺癌模型。 为未来的治疗研究奠定了基础。最后，表达 p21/Cip 1/Waf 1基因在雄激素依赖性和非依赖性前列腺中表达 将评估癌症样本。如果基础水平确实增加了 p21/Cip 1/Waf 1基因在雄激素非依赖性前列腺癌中的表达 观察到，它可以作为发展的临床标志物 雄激素依赖性前列腺癌的生长。

DESCRIPTION The prostate cancer is the second leading cause of cancer death in men. At early stage, the growth of prostate cancer is androgen-dependent and castration has been a very effective method in treating prostate cancer. Development of androgen-independent growth of prostate cancer after approximately 18 months of hormone ablation therapy is a major clinical dilemma. Goal of this study is to investigate the molecular mechanisms of CKI p21/Cip1/Waf1 in androgen-dependent and -independent growth of prostate carcinoma cells. This study is based on our observations that p21/Cip1/Waf1 gene was up-regulated by androgen and an increased basal expression of the gene was found in an androgen-independent prostate cancer cell line. This proposal will investigate the anti-apoptosis function of p21/Cip1/Waf1 gene in prostate carcinoma cells, which may confer the homeostasis of the cells in androgen-dependent manner and development of androgen-independent growth of pro-static cancer cell by increased basal expression of the gene. The molecular mechanism of androgen action on p21/Cip1/Waf1 gene expression will be elucidated. In addition, an in vivo androgen-independent prostate cancer model will be established for future therapeutic studies. Finally, the expression of the p21/Cip1/Waf1 gene in androgen-dependent and -independent prostate cancer specimens will be evaluated. If indeed an increased basal level of p21/Cip1/Waf1 gene in androgen-independent prostate cancer specimens was observed, it can be served as a clinical marker for developing androgen-dependent growth of prostate cancer.