Core--Immunology

核心--免疫学

• 批准号: 6228099
• 负责人: Malek Kamoun
• 金额: $ 23.33万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-28 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6228099
• 关键词: biomedical facility; cytokine; enzyme linked immunosorbent assay; flow cytometry; immunology

The Immunology Core will perform multi-parameter flow cytometry assays and cytokine assays. Dr. Kamoun, will be the Director of this core. He is the Director of the Clinical Immunology Laboratory at the University of Pennsylvania Medical Center. The Clinical Immunology Laboratory provides a wide range of laboratory tests on patients with autoimmune diseases including MS, and SLE as well as advanced multi- parameter four-color flow cytometry-based assays. Alterations in subset of CD4+ and CD8+ T cells and B cells can be determined in MS and SLE patients treated with antibody therapies. Lymphocyte activation can be evaluated assessing expression CD4+, CD8+ or B cells of a series of activation markers and adhesion molecules. Analysis of constitutive and induced cytokine production by intracellular staining of permeabilized cells can be performed using whole blood single cell cytokine assays which was recently established in our laboratory. This method allows the identification of cells producing cytokine while simultaneously analyzing the surface phenotype. In vitro production of cytokine can also be measured using ELISA-based assays. We can measured IL-2, IL-4, IL-10, IL-12 (p40 and the biologically active p70 heterodimer), INF-gamma, TNF-alpha and LT-alpha. All reagents used in the Laboratory are subjected to quality control procedures prior to use. All results of QC procedures are reviewed by the supervisor with corrective action implemented as necessary. Our lab participates in QA programs including the CAP surveys quarterly and in the CDC T cell immunophenotyping performance evaluation program. Performance of the flow cytometers is monitored daily using a suspension of beads to demonstrate scatter, fluorescence, and compensation parameters within defined limits. The Technologists employed in this section of the laboratory are each qualified under CLIA88 guidelines to perform "high complexity testing". The Laboratory Director and the Supervisor meet CLIA 88 requirements as director and technical supervisor respectively. The laboratory maintains a comprehensive safety program for all employees encompassing OSHA regulation and conforming to all Federal, State and local regulation.

免疫学核心将进行多参数流式细胞术测定和细胞因子测定。 Kamoun 博士将担任该核心的主任。他是宾夕法尼亚大学医学中心临床免疫学实验室的主任。临床免疫学实验室为患有自身免疫性疾病（包括多发性硬化症和系统性红斑狼疮）的患者提供广泛的实验室测试，以及基于先进的多参数四色流式细胞术的检测。可以在接受抗体疗法治疗的 MS 和 SLE 患者中确定 CD4+ 和 CD8+ T 细胞和 B 细胞亚群的变化。可以通过评估一系列激活标记物和粘附分子的 CD4+、CD8+ 或 B 细胞的表达来评估淋巴细胞激活。可以使用我们实验室最近建立的全血单细胞细胞因子测定法，通过透化细胞的细胞内染色来分析组成型和诱导型细胞因子的产生。该方法可以鉴定产生细胞因子的细胞，同时分析表面表型。细胞因子的体外产生也可以使用基于 ELISA 的测定法进行测量。我们可以测量 IL-2、IL-4、IL-10、IL-12（p40 和生物活性 p70 异二聚体）、INF-gamma、TNF-α 和 LT-α。实验室使用的所有试剂在使用前均经过质量控制程序。质量控制程序的所有结果均由主管审查，并根据需要采取纠正措施。我们的实验室参与 QA 项目，包括季度 CAP 调查和 CDC T 细胞免疫表型性能评估项目。每天使用珠子悬浮液监测流式细胞仪的性能，以证明散射、荧光和补偿参数在规定的限度内。实验室此部分雇用的技术人员均符合 CLIA88 指南的资格，可以执行“高复杂性测试”。实验室主任和主管分别符合 CLIA 88 作为主任和技术主管的要求。该实验室为所有员工制定了全面的安全计划，其中包括 OSHA 法规并符合所有联邦、州和地方法规。