Bacterial poly-histidine triad proteins

聚组氨酸三联体（Pht）蛋白是由链球菌属和其他革兰氏阳性属表达的表面蛋白的特征性较差的家族。最近的研究表明，Pht蛋白在这些细菌在低锌（Zn）环境中的生存中起着重要作用。该项目的假设是，Pht蛋白特异性地从细胞外环境中募集Zn，并以某种方式使其可用于位于细胞壁下方的细菌质膜中的ATP结合盒（ABC）转运系统，从而促进细菌对Zn的吸收。该项目的目的是进行全面的分子表征Pht蛋白，锌和ABC转运蛋白之间的相互作用，以及组氨酸三联体基序在这些相互作用中的作用。

The poly-histidine triad (Pht) proteins are a poorly characterised family of surface proteins expressed by the genus Streptococcus and other Gram-positive genera. Recent studies suggest an important role for Pht proteins in survival of these bacteria in low zinc (Zn) environments. The project hypothesis is that Pht proteins specifically recruit Zn from the extracellular environment and somehow make it available to ATP binding cassette (ABC) transport systems located in the bacterial plasma membrane, beneath the cell wall, facilitating Zn uptake by the bacterium. The aim of this project is to conduct comprehensive molecular characterization of the interactions between Pht proteins, Zn and ABC transporters, and the role of the histidine triad motifs in these interactions.