MOLECULAR TYPING OF RECURRENT CHLAMYDIAL STDS

描述(改编自申请人的摘要)：沙眼衣原体 是发达国家性传播疾病(STD)的主要原因 世界。这些感染大多是亚临床和非急性的。 占大量输卵管疾病妇女的主要原因 今天非常普遍的不孕不育和宫外孕。此外， 高达50%的青少年和年轻成年女性会再次感染 衣原体感染会造成输卵管疤痕。因此，青少年是一种 分析传播模式和类型的重要目标群体 以设计适当的干预策略。 菌株分型对于确定猪传染性支气管炎的分子流行病学至关重要。 不同衣原体感染背景下的衣原体感染 无症状和有症状的疾病状态，包括尿道炎， 子宫颈炎、盆腔炎和反复感染。 主要外膜蛋白(MOMP)的单抗。 沙眼衣原体历来被用来打字。然而，血清型却没有。 反映了在基因和蛋白质水平上发现的相同程度的变异 这种有机体。OMPL基因分型已经发展起来，它基于 MOMP基因(Omp1)。它能够检测到当前新的亚型 已知的血清型，为免疫分型提供了一种强大的替代方案。 基因分型是评估传播模式和 反复感染，并用作行为的生物衡量标准 与行为相关的研究和公共卫生监测。 具体目标：1)确定青少年中的ompl基因类型和变异。 在感染力和行为变量上存在差异的男性和女性； 2)分析ompl基因分型在伴侣和 在青少年群体内；3)评估发生在 青少年与首次(幼稚)或以前的感染相比，定义 在这些时间点内在OMPL中发生的突变类型，并评估 基因多态、共病原体和免疫反应在发病中的作用 宫颈和尿路部位的再感染；以及4)定义分子 可能与临床和临床相关的基因类型的特征 微生物学数据；分析序列-结构/功能关系 来自原型株和变异株的MOMP。

DESCRIPTION (Adapted from the applicant's abstract): Chlamydia trachomatis is the leading cause of sexually transmitted diseases (STD) in the developed world. The majority of these infections are subclinical and non-acute in presentation which accounts for the large number of women with tubal infertility and ectopic pregnancies that are so prevalent today. Further, up to 50% of adolescent and young adult women become reinfected with Chlamydia which contributes to tubal scarring. Thus, adolescents are an important target group in which to analyze transmission patterns and types of recurrence in order to design appropriate interventional strategies. Strain typing is essential for defining the molecular epidemiology of chlamydial infections within the context of different chlamydial asymptomatic and symptomatic disease states that include urethritis, cervicitis, pelvic inflammatory disease, and recurrent infection. Monoclonal antibodies to the major outer membrane protein (MOMP) of C. trachomatis have historically been used for typing. Yet, serotypes do not reflect the same degree of variation found at the gene and protein level of this organism. ompl genotyping has been developed which is based on the MOMP gene (ompl ). It is capable of detecting new subtypes of currently known serotypes and provides a powerful alternative to immunotyping. Genotyping is an appropriate tool for evaluating transmission patterns and recurrent infections, and for use as a biologic measure of behavior in behavioral related studies and for public health surveillance. Specific Aims: 1) define the ompl genotypes and variants among adolescent males and females who differ by force-of-infection and behavioral variables; 2) analyze transmission patterns of ompl genotypes between partners and within teen groups; 3) evaluate types of recurrent infections that occur in adolescents in comparison with first (naive) or previous infections, define types of mutations that occur in ompl over these time points, and evaluate the role of genotype polymorphism, co-pathogens, and immune response in reinfection at cervical and urethral sites; and 4) define molecular characteristics of genotypes that may be associated with clinical and microbiologic data; analyze the sequence-structure/function relationships of MOMP from prototype and variant strains.