SPERMATOGONIAL STEM CELL CULTURE AND TRANSPLANTATION

我们已经开发出一种方法， 一个有生育能力的男性和一个不育男性的生精小管。 转移后，供体细胞群建立正常的 精子发生在受体睾丸中，成熟的精子是 产生并发现于附睾。 在最成功的 移植，受体男性变得有生育能力，供体细胞 如果在雄性后代中发现单倍型。 我们采用了这种方法 为了证明精原干细胞可以冷冻保存， 使单个雄性生殖细胞永生不死。 此外，移植 大鼠睾丸细胞会在生精管中产生精子 免疫缺陷小鼠的小管并产生大鼠精子，这表明 异种精子发生在其他物种中也是可能的。 这 睾丸细胞移植技术提供了一个机会， 用男性生殖细胞进行的实验， 不可能的 在这个项目中，我们有三个具体目标。 1.到 增加供体细胞定殖和再增殖的效率 受体小鼠的生精小管。 2.在试管中生长， 增加睾丸干细胞的数量， 重新填充受体睾丸。 3.为了改变基因 精原干细胞的特性和恢复新的 子代单倍型。 这些研究将使精原细胞移植对 在生物学、医学和农业中有广泛的应用。 在 此外，获得的知识将有助于减轻某些形式的男性 帮助开发新的避孕方法。

We have developed a method that allows transfer of testicular cells from a fertile male to the seminiferous tubules of an infertile male. Following transfer, the donor cell population establishes normal spermatogenesis in the recipient testes, and mature spermatozoa are produced and found in the epididymis. In the most successful transplantations, the recipient male becomes fertile, and the donor cell haplotype if found in progeny of the male. We have used this approach to demonstrate that spermatogonial stem cells can be cryopreserved, thus making individual male germ lines immortal. In addition, transplanted rat testis cells will generate spermatogenesis in the seminiferous tubules of immunodeficient mice and produce rat spermatozoa, suggesting that xenogeneic spermatogenesis may be possible for other species. This testis call transplantation technique provides an opportunity to perform experiments with male germ cells that previously were difficult or impossible. In this project, we have three specific aims. 1. To increase the efficiency with which donor cells colonize and repopulate the seminiferous tubules of recipient mice. 2. To grow in vitro and expand the number of the testicular stem cells responsible for repopulating the recipient testis. 3. To modify the genetic characteristics of spermatogonial stem cells and recover the new haplotype in progeny. The studies proposed will make spermatogonial transplantation useful for a wide range of applications in biology, medicine and agriculture. In addition, the knowledge gained will help alleviate certain forms of male infertility and help in the development of new contraceptive approaches.