SPERMATOGONIAL STEM CELL CULTURE AND TRANSPLANTATION

我们开发了一种方法，允许将睾丸细胞从 有生育能力的雄性到不育雄性的生精小管。 移植后，供体细胞群体建立正常 受精者睾丸中的精子发生，成熟精子 在附睾体中产生和发现的。在最成功的 移植后，受者雄性变得有生育能力，而捐赠者细胞 如果在雄性后代中发现单倍型。我们已经使用了这种方法 为了证明精原干细胞可以超低温保存，因此 使单个雄性生殖系不朽。此外，移植的 大鼠睾丸细胞将在生精细胞中产生生精作用 免疫缺陷小鼠的小管和产生大鼠精子，这表明 异种精子发生在其他物种中也是可能的。这 睾丸召回移植技术提供了一种机会 用雄性生殖细胞进行的实验，以前很难或 不可能。在这个项目中，我们有三个具体目标。1.至 提高供体细胞定植和再繁殖的效率 受体小鼠的生精小管。2.在体外生长和 扩大睾丸干细胞的数量负责 重新填充受者的睾丸。3.修改基因 精原干细胞的特性及其新的修复 子代的单倍型。 建议的研究将使精原细胞移植对 在生物、医学、农业等领域有着广泛的应用。在……里面 此外，所获得的知识将有助于缓解某些形式的男性 不孕不育和帮助开发新的避孕方法。

We have developed a method that allows transfer of testicular cells from a fertile male to the seminiferous tubules of an infertile male. Following transfer, the donor cell population establishes normal spermatogenesis in the recipient testes, and mature spermatozoa are produced and found in the epididymis. In the most successful transplantations, the recipient male becomes fertile, and the donor cell haplotype if found in progeny of the male. We have used this approach to demonstrate that spermatogonial stem cells can be cryopreserved, thus making individual male germ lines immortal. In addition, transplanted rat testis cells will generate spermatogenesis in the seminiferous tubules of immunodeficient mice and produce rat spermatozoa, suggesting that xenogeneic spermatogenesis may be possible for other species. This testis call transplantation technique provides an opportunity to perform experiments with male germ cells that previously were difficult or impossible. In this project, we have three specific aims. 1. To increase the efficiency with which donor cells colonize and repopulate the seminiferous tubules of recipient mice. 2. To grow in vitro and expand the number of the testicular stem cells responsible for repopulating the recipient testis. 3. To modify the genetic characteristics of spermatogonial stem cells and recover the new haplotype in progeny. The studies proposed will make spermatogonial transplantation useful for a wide range of applications in biology, medicine and agriculture. In addition, the knowledge gained will help alleviate certain forms of male infertility and help in the development of new contraceptive approaches.