REGULATION OF DNA REPLICATION IN IRRADIATED CELLS

受辐射细胞中 DNA 复制的调节

• 批准号: 2894935
• 负责人: GEORGE E. ILIAKIS
• 金额: $ 23.84万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2894935
• 关键词: DNA damage; DNA replication; ataxia telangiectasia; biological signal transduction; cell cycle; cell line; genetic regulation; ionizing radiation; oncogenes; oncoprotein p21; posttranslational modifications; radiation genetics; radiation sensitivity; ribozymes; simian virus 40; transfection /expression vector; virus antigen

DESCRIPTION: (Adapted from the applicant's abstract): Checkpoints assure that progression from one cell cycle stage to the next proceeds only after previous events have been completed. The long-term goal is to elucidate the molecular mechanism of checkpoints activated by DNA damage in S phase, which results in down-regulation of DNA synthesis. The hypothesis to be addressed by this proposal is that radiation induces a signal transduction pathway that stalls DNA replication by inactivating factors involved in replication initiation. The goal is to identify the replication machinery targets and use the information gained to define the signal transduction pathway. An SV40 in vitro replication system will be used. All components needed have been purified and the SV40 large T antigen is the only non-cellular component. The specific aims include: 1) to study the post-translational modification and activity of RP-A; 2) to study the post-translational modification and activity of SV40 T antigen; 3) to study the contribution of ATM, p53 and p21 to signalling that can lead to modifications of RP-A and SV40 T antigen; and 4) to begin to purify novel proteins involved in signalling.

描述：（改编自申请人摘要）：检查点保证

项目成果

A rapid preparation of extracts for DNA replication in vitro.

快速制备用于 DNA 体外复制的提取物。

• 发表时间: 1999
• 期刊: Radiation research
• 影响因子: 3.4
• 作者: Wang,Y;Wang,H
• 通讯作者: Wang,H

Down-regulation of DNA replication in extracts of camptothecin-treated cells: activation of an S-phase checkpoint?

喜树碱处理的细胞提取物中 DNA 复制的下调：S 期检查点的激活？

• 发表时间: 1997
• 期刊: Cancer research.
• 作者: Wang,Y;Perrault,AR;Iliakis,G
• 通讯作者: Iliakis,G

Roles of replication protein A and DNA-dependent protein kinase in the regulation of DNA replication following DNA damage.

复制蛋白 A 和 DNA 依赖性蛋白激酶在 DNA 损伤后 DNA 复制调节中的作用。

• DOI: 10.1074/jbc.274.31.22060
• 发表时间: 1999
• 期刊: The Journal of biological chemistry
• 作者: Wang,Y;Zhou,XY;Wang,H;Huq,MS;Iliakis,G
• 通讯作者: Iliakis,G

An ATM-independent S-phase checkpoint response involves CHK1 pathway.

独立于 ATM 的 S 期检查点反应涉及 CHK1 通路。

• 发表时间: 2002
• 期刊: Cancer research
• 影响因子: 11.2
• 作者: Zhou,Xiang-Yang;Wang,Xiang;Hu,Baocheng;Guan,Jun;Iliakis,George;Wang,Ya
• 通讯作者: Wang,Ya

Inhibition of DNA replication by tirapazamine.

• 发表时间: 2001-07
• 期刊: Cancer research
• 影响因子: 11.2
• 作者: Katherine B. Peters;Hongyan Wang;J. Brown;G. Iliakis